| Objective:The study is conducted to explore the association between the c.212-37 ins C(rs3832879) polymorphism in intron 1 in Fibroblast growth factor 23,FGF23 gene and Kawasaki Disease(KD)and Coronary Artery Lesion(CAL) by detecting it's distribution in children.The relevance has been studied in other countries.But the association between SNP rs3832879 in FGF23 gene and KD susceptibility has not been reported in Han population.This study was designed to investigate the association of the FGF23 gene SNP rs3832879 with KD susceptibility and the relationship between CAL in Han children. Methods:A case-control study was performed. The case group included 40 children who were diagnosed as KD between October 2010 and August 2013 in the Tianjin Children's Hospital. Twenty-six age and gender-matched children who came from clinical department were enrolled as the control group. All KD patients were divided into coronary artery lesion(CAL) group(n=14) and no coronary artery lesion(NCAL) group(n=26).All the study subjects are Chinese Han children. Genomic DNA was prepared from peripheral venous blood(2m L) using a genomic DNA isolation reagent kit. Polymerase chain reaction(PCR) and gene sequence analysis were applied to detect the single nucleotide polymorphism of FGF23 gene rs3832879. Results:1. Out of KD patients,16 cases(40%) have coronary artery lesions.The age of CAL varied from 2 months to 6 years. 8 cases(50%)~1 years old,3 cases(18.75%)~2 years old, 2 cases(12.5%)~3 years old, more than 3 years old,3 cases(18.75%),the average age is 2.01+1.62; 24 cases(60%) have no coronary artery lesions. The age of NCAL varied from 5 months to 6.25 years.~1 years old,1 cases(4.17%),6 cases(25%)~2 years old, 5 cases(20.83%)~3 years old, more than 3 years old,12 cases(50%), the average age is 2.27+1.57.2. Out of 16 cases with CAL patients,10 cases were boys and 6 cases were girls,with a ratio of them was 1.67:1; Out of 24 cases were NCAL patients,12 cases were boys and 10 cases were girls,with a ratio of them was 1.20:1;3. Out of 16 cases with CAL patients,9 cases were single coronary artery lesions(56.25%), 5 cases were bilateral coronary artery lesions.(31.25%), 2 cases were CAA.4. FGF23 gene SNP rs3832879 was detected in 20 subjects(30.30%).Among 40 children with KD 14(35%) patients carried the polymorphism of c.212-37 ins C(rs3832879) in FGF23 gene,among 26 children in the control group 6(23.08%) carried the FGF23 polymorphism There is no statistical significant differences between the polymorphism of c.212-37 ins C(rs3832879) in FGF23 gene distributions(?2=1.06,p = 0.30) of the two groups, C base was linked an increased risk for KD susceptibility(OR=1.79, 95%CI=0.59-5.50).5. Among 16 patients in CAL group 9(56.25%) carried the FGF23 polymorphism at this locus among 24 patients in non-CAL group 5(20.82%) carried such polymorphism There is a statistically significant difference between the polymorphism of c.212-37 ins C(rs3832879) in FGF23 gene distributions(?2=5.29, p =0.02) of the two groups, C base increased risk for CAL in Chinese Han children with KD(OR=4.89, 95%CI=1.21~19.71).6. Out of 16 cases with CAL patients,9 cases have the insert C base,2 cases(22.22%) were CAA,3 cases(33.33%) were single coronary artery lesions,4 cases(44.44%) were bilateral coronary artery lesions. Conclusions: 1. The independent risk factors attributed to the combination of CAL may be the patients who are younger than 1 years old. 2. Polymorphisms of FGF23 gene SNP rs3832879 may be associationed with CAL in Chinese Han children with KD. 3. C base of FGF23 gene SNP rs3832879 is thought to increase risk of developing CAL in Chinese Han children with KD(OR=4.89). 4. C base of FGF23 gene SNP rs3832879 may be a risk factor of developing KD(OR=1.79). 5. C base of FGF23 gene SNP rs3832879 may be associated with the degree of CAL. |
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