The Inerstigation Of Isatin On Behavior Despair Animal Models In Mice

Objective:Isatin is a widely distributed in plants, animals and the human body's important natural products found in the human body in recent years endogenous active substances, with a variety of pharmacological activities, their anti-Parkinson's disease (PD), anti-aging, lowering blood pressure, epilepsy and neurological protection and other aspects of the activity is very important for clinical significance. In this study, observed through the establishment of kindling model of 2,3-isatin effect of anti-fire and control the use of classical antidepressants, study its antidepressant effect.Methods:Amygdala kindling experiments, PTZ kindled mice experiments observation semicarbazide 2,3-isatin effect of anti-fire and the use of classical antidepressants amitriptyline as a positive control drug, study the antidepressant effect.Results:We successfully established the amygdala kindling model, observed that the ISA on amygdala kindling in rats significantly inhibited. ISA ip (50～200) mg·kg-1, increased significantly after the discharge threshold and reduce the onset of the degree; same section V of the reaction rate was significantly lower,100 mg·kg-1 dose can be reduced 89%. However, the role of 200 mg9kg-1ISA but less than 100 mg·kg-1 group, the mechanism is unclear. In addition, ISA discharge after the discharge latency and duration had no significant effect, even after the discharge process to extend the trend, indicating that ISA can not stop the spreading of abnormal electrical activity of the inhibitory effect of amygdala kindled epileptic focus increased post-discharge system threshold due.In the forced swimming test in the West Astragalus gum given to mice with ISA, compared amitriptyline in mice, the shortest average time to swim, there is no visible glue West Astragalus antidepressant effect, but the West does not gel with the experimental Astragalus The other drugs reaction can be considered to test a difference in swimming time is the ISA, the role of amitriptyline. ISA mice given the average time to swim than to give the average amitriptyline swimming a long time, that the mental state of ISA in mice produced stronger effects than amitriptyline, amitriptyline is used clinically Drug treatment of depression, compared from the ISA and amitriptyline dose relations, the results show that the ISA to have some antidepressant effects in mice. Conclusions:ISA with anti-light the role of antidepressants in mice has some effect on the development of natural anti-depressant drugs have potential applications.