Expression Of PD-1/PD-L1 On The Lymphocytes From Peripheral Blood And Its Clinical Significance In Type 2 Diabetes Disease

Type 2 Diabetes Melitus is a chronic disease and its pathogenesis is complex and is not clear at present but it is certain that the insulin resistance and insulin secretion defect are the main links in the incidence of diabetes. Previous study showed PD-1-PD-L1 path was involved in the development of autoimmune diseases. In this study we manifest the correlation of PD-1/PD-L1 expression level and clinical features of patients with Type 2 Diabetes Melitus through detecting the PD-1/PD-L1 expression in T cell subsets and monocytes in peripheral blood of patients with Type 2 Diabetes Melitus. Expression of PD-1 in the T lymphocytes from Peripheral Blood and Its Clinical Significance in Type 2 Diabetes MelitusObjective: To detect the expression level of PD-1 in T cell subsets in peripheral blood of patients with Type 2 Diabetes Melitus, analysis the correlation of PD-1 expression level and clinical features of patients with Type 2 Diabetes Melitus, in order to preliminarily study the role of PD-1 in Type 2 Diabetes Melitus. Method: The expression level of PD-1 inT cell subsets in peripheral blood of 70 patients with Type 2 Diabetes Melitus and 30 healthy persons was detected by immunofluorescence staining and Flow Cytometry, and the ratio of CD4~+ /CD8~+ T cells, the percent of PD-1~+CD4~+T cells, the percent of PD-1~+ /CD8~+ T cells and the percent of PD-1~+CD14~+cells was calculated for Correlation analysis. The correlation analysis between the percent of CD4~+PD-1~+ T cells and clinical characterizations in Type 2 Diabetes Melitus were carried out. 70 patients with Type 2 Diabetes Melitus were diveded into two groups by Body Mass Index: Obese group and Non-obese group. The correlation analysis between the percent of CD4~+PD-1~+ T cells and clinical characterizations in Type 2 Diabetes Melitus were then carried out.Result: (1) In diabets melitus group, the ratio of CD4~+/CD8~+ T cells, the percent of CD4~+PD-1~+T cells, the percent of PD-L1~+CD14~+ cells were higher than that in health group while the percent of CD8~+PD-1~+T cells has not changed significantly. (2)In diabets melitus group, the percent of CD4~+PD-1~+ T cells was positively correlated with the HOMA-IR, and has no correlation with 0'FINS ,30'FINS, TC and TG. In Obese group, the percent of CD4~+PD-1~+ T cells was higher than that in Non-obese group. (3)The percent of CD4~+PD-1~+ T cells in Obese group was positively correlated with theHOMA-IR and has no correlation with 0'FINS, 30'FINS, TC and TG; in Non-obese group, the percent of CD4~+PD-1~+T cells has no correlation with 0'FINS, 30'FINS, HOMA-IR,TC and TG..(4)One year later, the expression level of CD4~+ T cells, CD8~+ T cells, CD4~+PD-1~+ T cells, CD8~+PD-1~+ T cells and CD14~+PD-L1~+ cells has not changed significantly.Conclusion: The expression level of CD4~+PD-1~+ T cells and CD14~+PD-L1~+ cells in patients with Type 2 Diabetes Melitus was higer than that in control group, and positively correlated with clinical features, hinted that The negative costimulatory molecules PD-1/PD-L1 might involved in the development of Type 2 Diabetes Melitus. The intervention of PD-1/PD-L1 path may be benificial to improve the pathological process and insulin resistance in patients with Type 2 Diabetes Melitus.