The Roles Of Costimulatory Molecules CD80 And CD86 In Experimental Allergic Neuritis

Objective To investigate the roles of costimulatory molecules in experimental allergic neuritis(EAN).Methods EAN modles were established on 18 male New Zealand white rabbits. They were randomly divided into 3 groups. Subcutaneous injection took place on the rabbits of FKG with antigen emulsion that was mixed by FCA, KLH and GM1. FCA and KLH in FK group, FCA and GM1 in FG . There were 6 rabbits in each group and 3 injections on each rabbit. Lymphocyte and blood serum were collected before the first injection and every 10 days after the first injection. We analyse the expression of CD80 and CD86 mRNA in peripheral blood lymphocyte by Reverse Transcription Polymerase Chain Reaction(RT-PCR) and detect anti-GM1-IgG antibody titre in peripheral blood serum by Enzyme-linked immunosorbent assay (ELISA).Results The expression of CD80 mRNA in FKG and FK began to rise on the 10th day after immunity .It reached the peak on the 40th day after the injection.In FKG and FK it had a significant difference at each time point comparied with FG (P<0. 01),but there was no difference between FKG and FK(P>0.05). The expression of CD86 mRNA in FKG and FK began to rise on the 10th day after immunity .It reached the peak on the 30th day after the injection. In FKG and FK it had a significant difference at each time point comparied with FG (P<0. 01), between FKG and FK there was different only on the 40th day(P<0. 05),on the other time point there was no difference (P>0.05). Anti-GM1-IgG antibody titre in FKG began to rise on the 10th day after immunity. It reached the peak on the 40th day after the injection. In FKG it had a significant difference at each time point comparied with FK and FG (P<0. 01), but between FK and FG there was no difference (P>0.05).Conclusion Costimulatory molecules CD80 and CD86 play an important role in the activation of T cell mediated by protein carrier, then induced the production of anti-GM1 antibody, and then induced EAN..