The Research Of Nf-kB,TNF-a And PLGF In The Pathogenesis And Severity Of Preeclampsia And Theirs Relevance

Objective: To explore the role and relationship of the nuclear factor kappa B (NF-κB), tumor necrosis factor-alpha (TNF-a) and placental growth factor (PLGF) in the pathogenesis of preeclampsia, and further elucidate the etiological factor and pathogenesis of the preeclampsia and its value in clinical prediction. Methods: Forty normal pregnancy women (normal pregnancy group) and eighty preeclampsia patients (preeclampsia group) which include forty mild and forty severe preeclampsia patients were selected randomly. Immunohistochemistry staining was used to detected the expression of NF-κB,TNF-a and PLGF in placenta of normal pregnancy group and preeclampsia group. The level of TNF-a and PLGF in serum of normal pregnancy group and preeclampsia group was examined by the enzyme-linked immunosorbent assay (ELISA).The relevance of them was analyzed. Results: 1) Compared with the normal pregnancy group, the expression of NF-κB and TNF-a was remarkably stronger (P<0.01), and expression of PLGF was remarkably weaker (P<0.01) in the placenta in the preeclampsia group. The expression of NF-κB and TNF-a in the placenta in severe preeclampsia group was obviously stronger than in mild preeclampsia group (P<0.01), otherwise there was no statistical difference of the expression of PLGF between the two groups (P>0.05). 2) The expression of NF-κB and TNF-a was strikingly negatively correlated to that of PLGF (r=-0.7111, P<0.05, r=-0.8082, P<0.01), but the expression of NF-κB was strikingly positively correlated to that of TNF-a (r=0.9363, P<0.01), in the placenta in the preeclampsia group. 3) The level of TNF-a was remarkably higher (P<0.01), and the level of PLGF was remarkably lower (P<0.01) in the serum in the preeclampsia group than in the normal pregnancy group. The level of TNF-a was strikingly higher (P<0.05), and the level of PLGF was strikingly lower in the severe preeclampsia group than in the mild preeclampsia group (P<0.01). 4) The level of TNF-a was strikingly negatively correlated to that of PLGF in the serum of the preeclampsia group (r=-0.7002, P<0.01). Conclusions: The NF-κB,TNF-a and PLGF might take part in together in the pathogenesis of preeclampsia.they possibly interact and mutual regulate in the activation and injury of the endothelium of vascular. They are also have substantial connection with occurrence and development of preeclampsia and its severity. The level change of TNF-a and PLGF could be used as an important serum landmark, has very broad application prospects in clinical monitoring and treatment of preeclampsia.