| Preeclampsia (preeclampsia PE) is a pregnancy-specific maternal and child health aserious threat to common disease, in recent years, the leading causes of maternal, fetal andneonatal death. In recent years, the etiology and pathogenesis of preeclampsia has beenfurther studied, but its exact etiology and pathogenesis has not yet clearly, for the treatmentand prevention of diseases difficult. Given the symptoms of patients with preeclampsiaplacenta delivered quickly alleviated or cured the current accepted theory is relativelyshallow implantation of the placenta or placental defect mechanisms. But many scholarsbelieve it is the result of many factors in the mother, placenta, fetal and so on. The basicpathology of the disease is systemic small vessel spasm, vascular endothelial cell injuryand ischemia. Recent studies had found that vasoactive factors: Soluble Fms-like tyrosinekinase-1(sFlt-1), placental growth factor (PLGF) and soluble endothelial growth factor(sEng) are closely related to the development of preeclampsia. This paper studies thesoluble Fms-like tyrosine kinase-1(sFlt-1), placental growth factor (PLGF) and solubleendothelial growth factor (sEng) expression levels, as well as to study their correlationwith the occurrence of preeclampsia development.Objective：1. Soluble Fms-like tyrosine kinase-1(sFlt-1), placental growth factor (PLGF) andsoluble endothelial growth factor (sEng) expression levels, as well as to study theircorrelation with preeclampsia.2. Analyze the correlation between preeclampsia and oxidative stress, lipidmetabolism and inflammation mechanisms, providing new research directions for clinicaldiagnosis and treatment.Method:Selected group of45cases of preeclampsia according to the diagnostic criteria ofpreeclampsia, mild preeclampsia25cases,20cases of severe preeclampsia; and choose nopregnancy complications and complications in25cases of healthy pregnant women as acontrol group. Collection of preeclampsia and normal pregnant group peripheral blood8ml, take5ml blood for30minutes, then centrifuged at3000rpm for10minutes, draw the upperserum in EP tube, placed in-70℃refrigerator, enzyme-linked immunosorbent assay(ELISA) was used to detect preeclampsia and normal maternal serum sFlt-1, PLGF andsEng;3ml send another hospital laboratory detection TG, CHOL, HDL, LDL and CRP.With Microsoft office Excel2003to build a database of all subjects, using SPSS19.0statistical analysis of various indicators for comparisons between groups of pregnantwomen using the F-test, for any of a variety of indicators used to compare two groups ofpregnant women between q Inspection Act (Newman-Keuls method), the correlationbetween the two variables tested using Pearson correlation analysis to p<0.05wasconsidered statistically significant.Results:1. The average content of sFlt-1in the blood of patients with severe PE was6397.38±2667.53pg/ml, the average content in the blood of patients with mild PE was3628.30±1041.44pg/ml, the average content in the blood of normal pregnant women was2760.51±730.86pg/ml, sFlt-1levels in pregnant women in each group difference was statisticallysignificant (F=30.02, p<0.01). Upon examination found that patients with severe PE sFlt-1levels were more than normal pregnant women and patients with mild PE, and thedifferences were statistically significant (q=10.64,8.10, p<0.01); mild PE sFlt-1levels hadno significant difference in normal pregnant women (q=2.69, p>0.05).2. PLGF average content in the blood of patients with severe PE was122.26±55.38pg/ml, the average content in the blood of patients with mild PE was219.79±88.36pg/ml,the average content in the blood of normal pregnant women was326.27±113.97pg/ml,PLGF level differences between groups of pregnant women were statistically significant(F=27.86, p<0.01). Upon examination found, PLGF level in normal pregnant woman ishigher than patients with mild to severe PE, and the differences were statisticallysignificant (q=5.84,10.51, p<0.01); PLGF levels in patients with mild PE were also higherthan severe PE patients, the difference was statistically significant (q=5.00, p<0.01).3. SEng average content in the blood of patients with severe PE was23.8±6.5ng/ml,the average content in the blood of patients with mild PE was9.1±1.4ng/ml, the averagecontent in the blood of normal pregnant women was2.4±0.5ng/ml, sEng level differencesbetween groups of pregnant women was statistically significant (F=206.93, p<0.01). Uponexamination found, sEng levels in normal pregnant patients were lower than patients with mild to severe PE, and the differences were statistically significant (q=9.38,28.41, p<0.01);sEng levels in patients with mild were lower than severe PE patients,The difference wasstatistically significant (q=19.57, p<0.01).4. The average CRP levels in the blood of patients with severe PE was23.72±5.53mg/l, the average content in the blood of patients with mild PE was19.07±3.76mg/l,the average content in the blood of normal pregnant women was9.43±0.62mg/l, CRPlevels in pregnant women in each group difference was statistically significant (F=87.48, p<0.01). Upon examination found, CRP levels in normal pregnant patients were lower thanpatients with mild to severe PE, and the differences were statistically significant(q=12.88,18.04, p<0.01); patients with mild PE CRP levels were less than patients with severePE.The difference was statistically significant (q=12.88,18.04, p<0.01).5. TC average content in the blood of patients with severe PE was7.03±1.51mmol/l,the average content in the blood of patients with mild PE was6.23±0.54mmol/l, theaverage content in the blood of normal pregnant women was5.46±0.70mmol/l; TGaverage content in the blood of patients with severe PE was4.17±1.32mmol/l, the averagecontent in the blood of patients with mild PE was3.21±0.90mmol/l, in the normal Theaverage content of the blood of pregnant women was2.22±0.50mmol/l; LDL averagecontent in the blood of patients with severe PE was3.63±0.69mmol/l, the average contentin the blood of patients with mild PE was2.87±0.48mmol/l, in the blood of pregnantwomen in the normal average content was2.62±0.58mmol/l. Pregnant women in eachgroup TC, TG and LDL-Clevels had a statistically significant difference(F=4.77,24.26,17.39, p<0.01); TC, TG, LDL-C levels in patients with severe PE weresignificantly higher than normal pregnant women, the difference was statisticallysignificant (q=7.67,9.82,8.11, p<0.05); patients with severe PE, TC, TG, LDL-C levelswere significantly higher than patients with mild PE, the difference was statisticallysignificant (q=3.90,4.84,6.83, p<0.05); HDL-C levels in patients with severe PE werebelow the patients with mild PE, and also lower than normal pregnant women, thedifference was statistically significant,(q=4.20,3.61, p<0.05); in patients with mild PE TC,TG levels were significantly higher than in normal pregnant women, the difference wasstatistically significant (q=4.00,5.29, p<0.05); in patients with mild PE and normalpregnant women, HDL-C, LDL-C levels had no statistically significant difference (q=0.63, 0.519, p<0.01); sFlt-1, sEng and TG was a significant positive correlation (r=0.729,0.557,p<0.01); patients with preeclampsia serum PLGF,CRP and TG showed a significantnegative correlation (r=-0.729,-0.600, p<0.01).Conclusion:1. Patients with severe PE sFlt-1levels were higher than normal pregnant women andpatients with mild PE; Patients with mild PE sFlt-1levels and normal pregnant women hadno significant difference; PLGF levels in normal pregnant women were higher thanpatients with mild to severe PE, PLGF level in patients with mild PE is also higher thanpatients with severe PE; SEng levels in normal pregnant women were lower than patientswith mild to severe PE, patients with mild PE sEng levels were below severe PE patients.2. CRP levels in normal pregnant women were lower than patients with mild to severePE, CRP levels in patients with mild PE were lower than in patients with severe PE,indicating the presence of preeclampsia excessive inflammatory response, and with theprogress of the disease and worse; Patients with severe PE,TC, TG, LDL-C levels weresignificantly higher than in normal pregnant women and patients with mild PE, HDL-Clevels in patients with severe PE were less than patients with mild PE, and also lower thannormal pregnant women; in patients with mild PE,TC, TG levels were significantly higherthan in normal pregnant women, indicating the presence of preeclampsia fat metabolism,and also with disease progression and worse.3. Preeclampsia serum sEng and sFlt-1and CRP and TG was significantly positivelycorrelated; preeclampsia serum PLGF,CRP and TG was significantly negatively correlated.Showed that:①Oxidative stress, inflammation and lipid abnormalities may play asynergistic role in preeclampsia, intervention oxidative stress, inflammation anddyslipidemia may help control the occurrence and development of preeclampsia.②Combined diagnostic performance than a single diagnosis. |
PDF Full Text Request