Clinical And Experimental Research On Treating Diarrhea IBS Caused By Hepatic Stasis, Spleen Deficiency By Applying Baishi Decoction

The first department:clinical researchObjectives:TO observe the clicial effects in treating Irritable Bowel Syndrome(IBS) With diarrhoea caused by hepatie stasis, Spleen deficiency with the decoction of baishi. To discuss its therapeutic action mechanism from the theory and the clinical effects.Methods:This research was based on 60 cases of outpatients with hepatie stasis, spleen deficiency from the clinic of the hospital attributed to TCM of Jiangsu Province.They were randomly divided in to two groups.One group was the treatment group,30 patients of which were treated with the decoction of baishi.The other group was the control group, with 30 patients being treated with Pinaverium bromide.These patients all met the TCM diagnosis criterion. Both groups were treated for eight weeks.Clinical symptoms'changes before and after treatment were detected respectively to evaluate the therapeutic effects.Results:After one course of treatment, significant difference was apparent in the clinical effects:In the treatment group,11 cases recovered and 13 cases significantly improved,4 cases improved,2 ineffcetual cases.The total effective rate in the treatment group is 93.3%.In the control group 2 cases recovered and 8 cases significantly improved,11 cases improved,9 ineffectual cases.The total effective rate in the control group is 70.0%.There was statistically significant difference in the total effective rate between the treatment group and control group(p<0.01).The effective rate from the treatment group is clearly superior.Conclusions:Above all, the decoction of baishi appears to have the significant effect in the treatment of IBS with diarrhoea caused by hepatic stasis and spleen deficiency,which is is clearly superior to he control group. The results demonstrate the practicality and feasibility.The second department:experimental researchObjectives:To explore mechanism of the decoction of baishi in curing experimental rat model of Irritable Bowel Syndrome(IBS) With diarrhoea caused by hepatie stasis, spleen deficiency by constructing animal model and observing correlated indexes(the cells of MC and 5-HT).The aim is to provide experimental evidence for clinical research.Methods:26 healthy wistar rats were randomly divided into five groups, control group had 3 rats, western medicine group had 5 rats, the other three group each had 6 rats.Diarrheal rats were established by intragastric administration with senna apozem and restraint-stress.2 weeks later when the models were established, the amount of decoction of baishi given to rats in large dose group and low dose group was 35g/kg weight and 10.5g/kg weight respectively.The amount of Pinaverium bromide given to control group was 30mg/kg weight. Two weeks later,all rats were killed to observe following index:cells of MC and 5-HT in small intestine and pathology of bowel tissue.Results:Contracted to model group between the western medicine group and the herb of low dose and high dose after dyeing of HE, The improvement of intestinal inflammation of the herb has significant effect superior to medicine.Contracted to model group between the medicine group and the herb of low dose and high dose after dyeing of toluidine blue and trypsin, the herb group has statistically significant (p<0.01). Contracted to the control group, the herb group has no statistically significant (p>0.05)Contracted to the western medicine group and the herb of low dose and high dose after dyeing of c-fos, the herb group has statistically significant (p<0.01). Contracted to the control group, the herb group has no statistically significant (p>0.05), while the western medicine group and model group all have statistically significant (p<0.01)Contracted to model group between the western medicine group and the herb of low dose and high dose after dyeing of 5-HT, the three groups all have statistically significant (p<0.01)It indicated there were significant therapeutic effects of the herb of baishi on improvement of intestinal inflammation by inhibiting the activation of intestinal MC and reducing the expression of 5-HT of the rats.Conclusions:The decoction of baishi can improve the intestinal pathology of the rats of IBS-D.The possible mechanism may be related to improve the intestinal inflammation by inhibitting the activation of intestinal MC and reducing the expression of 5-HT of the rats,which can help to achieve therapeutic purposes.