Effect Of Acupuncture Treatment On The Clinical Trial And CGRP, NPY, And 5-HT Expression In The Rat Model About Irritable Bowel Syndrome

Purpose:Through clinical observation and animal research, to explore the effect of interference and the regulation mechanism of acupuncture on irritable bowel syndrome and the CGRP,5HT, NPY of the brain-gut interaction model.Methods:It's a single-blind randomized controlled clinical trial, and 117 patients with IBS were randomly divided into two groups. Treatment group with 56 patients were accepted with acupuncture, and the control group with 61 patients was treated with Pinaverium Bromide Tablets and Fiberform for symptomatic treatment. Symptom scores and VAS scores of abdominal pain or discomfort before and after treatment were compared to evaluate its clinical effect; this research is to build and re-evaluate the brain-gut interaction model of IBS with the slow acute joint stress,16 of the successful models were randomly selected as the treatment group with EA therapy. After the treatment, the role of acupuncture was assessed with its general behavior and the detection of levels of CGRP,5HT, NPY in the hypothalamus, spinal cord (L4-L6) and distal colon among three groups.Results:①Symptom scores were compared, the treatment group score have been lower than the control group (p<0.05) since the first week.1 month after the treatment, both groups score increased than the score after treatment(P<0.05); the total effective rate of treatment group was 90.0%, better than the drug control group 84.0%(p<0.01), both clinical cure rates are low.②After the modeling and evaluation, the result have confirmed that the model established with abnormal intestinal motility, visceral hypersensitivity and psychological behavior change, and there is no obvious intestinal histopathological abnormalities occur, which suggest modeling success.③After the treatment with EA for 2 weeks, stool particles for 1 hour, syrup intake and CRD threshold of treatment group were significantly different from the model group (P<0.01), similar number of the control group (P>0.05); the levels of 5-HT, CGRP and NPY in the colon, hypothalamic and spinal cord specimens of model group were abnormal (P<0.05), while the levels of the treatment group were similar to the control group (P> 0.05).Conclusion:①The acupunctrue therapy for IBS produce faster effect and have more obvious effects with the longer course of treatment, can significantly reduce abdominal pain or discomfort. After the treatment, patients often have got the IBS symptoms again, but acupuncture still have better long-term effect than drug treatment.②Electroacupuncture can regulate the symptoms caused by intestinal motility disorders, psychological and behavioral abnormalities and visceral hypersensitivity on the brain-gut interaction rat model of irritable bowel syndrome.The 5-HT, CGRP and NPY of the brain-gut axis were closely related with the IBS symptoms, while acupuncture can regulate the abnormal expression of 5-HT. CGRP and NPY, so as to restore the balance and to get good effect on IBS.