The Expression And Significance Of AQP1 In Adenomyosis

ObjectiveInvestigating AQP1 in ectopic endometrium and eutopic endometrium of adenomyosis, indentify its effect on pathogenesis of adenomyosis.MethodsSamples were collected between January 2007 and December 2009 at Gynecology of Tianjin First Center Hospital. Eutopic and ectopic lesions were taken from 40 patients who undergone the whole hysterectomy or subtotal hysterectomy and confirmed pathologically as adenomyosis and no merger with pelvic endometriosis, ovarian endometriosis, hysteromyoma and gynecological malignancy. The age of this group was between 34-54 years old. Normal endometrim is obtained with exploratory curettage from 21 patients who suffer ovarian benign tumor and no merger with abnormal uterine bleeding and confirmed pathologically. The age of this group was between 24-46 years old.Requirements for the sample population included:no steroid treatment, if medical treatment was used, it should be stopped for 6 months before surgery; no intrauterine device for 6 months before surgery. Immunohistochemical method is used to detect expression of AQP1 in eutopic endometrium, ectopic endometrium and normal endometrium.ResultsAQP1 can be detected in ectopic endometrium, eutopic endometrium and normal endometrium. Positive signal is mainly located in the cytoplasm of interstitial microvascular endothelial cell in endometrim.①No obvious difference is confirmed between proliferation (6.19±1.15) and secretory (7.43±2.52) period in normal endometrim (t=1.23, p>0.05).②No obvious difference is confirmed between proliferation (16.49±6.47) and secretory (17.65±6.00) period in eutopic endometrium of adenomyosis (t=0.59, p>0.05).③AQP1 in ectopic endometrim (33.52±8.27) is higher than eutopic endometrim (17.07±6.21)(t=10.06, p<0.05).④AQP1 in eutopic endometrim is higher than normal endometrim (7.02±2.21) (t=9.19,p<0.05).⑤AQP1 in ectopic endometrim is higher than normal endometrim (t=19.01,p<0.05). ⑥AQP1 in eutopic endometrim of adenomyosis and clinical characteristics:No obvious difference is confirmed between dysmenorrheal (16.69±6.11) and no dysmenorrheal (17.63±6.51) (t=0.46,p>0.05). No obvious difference is confirmed between menorrhagia (16.71±5.47) and no menorrhagia (17.51±7.25) (t=0.40,p>0.05). No obvious difference is confirmed between hysterauxesis (17.03±6.91) and no hysterauxesis (17.17±3.64) (t=0.06,p>0.05). No obvious difference is confirmed between history of uterine operation (17.24±6.64) and no history of uterine operation(16.67±5.32)(t=0.26,p>0.05).AQP1 in ectopic endometrim of adenomyosis and clinical characteristics:No obvious difference is confirmed between dysmenorrheal (34.42±9.58) and no dysmenorrheal (31.42±6.68) (t=1.09,p>0.05). No obvious difference is confirmed between menorrhagia (32.65±6.90) and no menorrhagia (34.67±9.95) (t=0.76,p>0.05). No obvious difference is confirmed between hysterauxesis (33.70±8.99) and no hysterauxesis (32.97±5.97) (t=0.24,p>0.05).No obvious difference is confirmed between history of uterine operation (33.53±9.19) and no history of uterine operation(33.47±5.95)(t=0.02,p>0.05).ConclusionAQP1 in ectopic endometrium and eutopic endometrium are both higher than normal endometrium, suggesting that AQP1 have certain effect in the occurrence and development of adenomyosis.AQP1 in ectopic endometrium is higher than eutopic endometrium, the reason may be inflammatory mediators in ectopic endometrium can raise the AQP1 expression.