Expression Of CLEC-2BmRNA And The Relationship With The Clinical Of Vitiligo

ObjectiveWe discussed the relationship between the CLEC-2B (C-type lectin domain family 2, member B) gene in vitiligo lesions and in the peripheral blood of patients with the different clinical types of disease expression, course, stage of disease, outcome relevance, in order to discover the diversity of disease and clinical outcome and the relevance of differential gene expression and to predict vitiligo, clinical type, stage and prognosis, Thus which plays a role in guiding the clinical. And using the immunohistochemical methods to detect the location expression of the lesion area CLEC2B protein, we can clarify the cellular localization of CLE-C2B.MethodsUse reverse transcription - polymerase chain reaction method(RT-PCR), analysise vitiligo patients and normal controls on the peripheral blood and skin lesions of CLEC-2B expression, PCR products were 4.0% agarose ethidium bromide electrophoresis, and then certified under the camera gel imager. According to the diagnostic criteria of vitiligo, use statistical software to analyze the CLEC-2B gene expression differences of the different clinical forms, different period, different course, prognosis of vitiligo lesions in different areas, non-lesion area, peripheral blood and normal skin, peripheral blood. The vitiligo lesions which the CLEC-2B transcript expression is significantly higher than in the organization taken, and use the immunohistochemical methods to detect the location expression of the lesion area CLEC-2B protein.Results1. The patients with vitiligo lesions in the CLEC-2BmRNA expression was significantly higher than the normal control group (t=2.581,P<0.05).2. The CLEC-2BmRNA expression of vitiligo patients compared with normal control group has no significant difference (t=1.375,P> 0.05).3. The expression of CLEC-2BmRNA in Vitiligo lesions group of men and women has no significant difference (t=1.341,P> 0.05).4. CLEC-2BmRNA in patients with different course of the expression of vitiligo has no significant difference (F=1.479,P> 0.05).5. vitiligo vulgaris group of CLEC-2BmRNA expression was significantly higher than that Segmental vitiligo, there were significant differentce(t=2.432, P<0.05).6. The expression of CLEC-2BmRNA in advanced lesions with vitiligo higher than patients with stable, the differences were statistically significant (t=2.204,P <0.05).7. In the different outcome of vitiligo patients, the difference has statistically significant (F=5.218,P<0.05).8. Confirmed by immunohistochemistry, there is CLEC-2B gene expression in the true level of lymphocytes of vitiligo lesions.Conclusions1. The CLEC-2B mRNA expression levels of vitiligo patients with lesions increased, which imply that the CLEC-2B in the pathogenesis of vitiligo has a certain significance.2. Vitiligo patients with advanced CLEC-2B was significantly higher than stable patients, it tips that it has association between CLEC-2B and stage of disease progression of vitiligo, which is as to determine the progress indicators of vitiligo.3. CLEC-2B in the expression of segmental vitiligo was significantly higher than that of vitiligo vulgaris, that is to say, the CLEC-2B gene can decide the type of vitiligo, meanwhile, it is speculated that CLEC-2B may be involved in immune mechanisms of vitiligo.4. The CLEC-2B expression difference between the different outcomes of vitiligo patients has statistically significant, which revealed the same disease at the genetic level of the mystery of different outcomes, to guide clinical diagnosis and prognosis. At the same time to search for drugs that regulate the gene, and lay the foundation for improved disease outcome.5. The CLEC-2B gene express in the skin lesions of patients with vitiligo of lymphocytes in the dermis.