Study On Tissue Culture And Physiological And Biochemical Basis Of Organ Formation Of Phalaenopsis

An effective way was established to rapidly Phalaenopsis by induction of bud clumps after a series of studies on the disinfecting methods of explant,medium types,hormone concentration and browning control. Meanwhile, the physiological and biochemical changes in various stages of organ differentiation of Phalaenopsis was basically researched. The results were as follows:1.Rapid propagation technique of PhalaenopsisTakeing Phalaenopsis V31 at full-bloom stage as explants were established a sterile system. The best formula of medium types to directry induce cluster buds from the explant of the upper stem was: 1/2 MS + 6-BAP 5.0mg/L + NAA 0.1mg/L + sucrose 30g/L + agar 7.0g/L,pH5.4. For the explant of the base of the stem segments, the best indirectly induction medium of cluster buds was: 1/2MS+6-BAP 5.0mg/L+NAA (0.1,0.5)mg/L+sucrose 30g/L+agar7.0g/L, pH5.4. For the explant of seeds embryo, the best medium of protocorm induction was 1/2MS+tryptone 0.5g/L+banana mud 30ml/L+sucrose 30g/L+agar 7.0g/L,pH5.4. The best rooting medium: 1/2MS+banana mud 30ml/L+ctivated arbon 1g/L+ tryptone0.5g/L+sucrose 30g/L+agar7.0g/L, pH5.4. The best way of transplanting acclimation: closing outdoor for 3d and then opening outdoor for 3d, the tissue culture seedlings were disinfected with 0.1% carbendazim for 5min,then planted on the moss which was disinfected with 0.1% agricultural streptomycin for 2d.2. Basic research of organ differentiation in physiology and biochemistry during the process of tissue culture regeneration in PhalaenopsisDuring the process of tissue culture in Phalaenopsis, physiological indicators such as endogenous hormones(IAA,GA3,ZR,ABA) soluble sugar content,soluble protein content changed regularly, and this change was related with different organ formation closed. Different relevant proteins appeared on different stages of organ formation during the process of tissue culture of Phalaenopsis, and they had different expression ways. The elective expression of these relevant proteins forced the regeneration of different organ.