Study On Tissue Culture And Rapid Propagation Technology Of 8 Wild Lilies

Lilium spp.known as the "king of bulbous flowers",is one of the world’s five most giant fresh-cut flowers.Lily has not only high ornamental value,but also edible and medicinal value.They are the first batch of dual-use plants approved by the Ministry of Health of China.They are widely used in clinic and have great development prospects as raw materials for processing health products.Although China is the leading natural distribution center of wild lilies,the commercial cultivation of ornamental lilies started relatively late,and the breeding and propagation need to catch up.In addition,most of the lily bulbs are now imported from Japan,the Netherlands,and other countries.The lily bulbs localization is still facing great difficulty,which has been the bottleneck of China’s commercialized lily production.Because of this,it is essential to use modern breeding techniques like genetic transformation to study how to breed lilies more efficiently and improve their qualities.Traditional flower propagation methods include sowing,cutting,splitting,layering and grafting.However,with the flower industry’s rapid development,tissue culture propagation technology is increasingly applied.Although the bulb breeding technology of lilies is becoming more mature,the large-scale production of lily bulbs and cut flowers is still restricted due to its slow speed and low reproduction coefficient.Cultivation lily tissue culture seedlings has the advantages of a high propagation coefficient and a short breeding cycle,which make up for the deficiencies of seed ball breeding.In recent years,people have found many rare and unique lily species.Wild species have their own characteristics,which greatly value research and development.They are also excellent materials for lily breeding.The author established and optimized an efficient regeneration system for eight wild lily species as research materials;Using L.lophophorum,L.wenshanense,L.distichum,L.brownii,L.bakerianum,L.papilliferum,L.pumilum,and L.lankongense as experimental materials,the characteristics of these eight lilies are completely different,There are indirect and direct ways of plant regeneration between lilies.In order to reduce the occurrence of variation,this experiment used the scales of L.lophophorum,L.wenshanense,L.distichum,and L.brownii as explants,and used the tissue culture seedlings of L.bakerianum,L.papilliferum,L.pumilum,and L.lankongense as materials,To explore the effects of different disinfection methods on the survival of lily,and to explore the effects of different hormone combinations on the induction,proliferation,and rooting of adventitious buds from lily scales;The best culture medium was selected in order to lay the foundation for the conservation and efficient breeding of rare and unique lily germplasm resources,as well as the construction of an in vitro conservation system using excellent wild lily materials.Through efficient propagation of small bulbs,the adventitious buds formed from small bulbs can achieve the goal of rapid propagation.The main results are as follows:(1)The optimal disinfection method for L.wenshanense is as follows: 75% alcohol for 60 s + 2% sodium dichloroisocyanurate for50min+1 drop of Tween 20;the optimal induction medium is MS +0.2mg/L NAA + 0.5-1.0mg/L TDZ;The optimal medium for proliferation and rooting is MS + 30g/L sucrose + 4g/L gellan gum;The optimal medium for callus induction is MS+3.0mg/L PIC + 1.0mg/L NAA +0.5mg/L TDZ.(2)The optimal disinfection method for L.distichum is: 75% alcohol for 60 s + 2% sodium dichloroisocyanurate for 50 min +1 drop of Tween20;The optimal induction medium was MS + 30g/L sucrose + 4g/L gellan gum,with an induction rate of 73.33% and the fastest healing time;The optimal proliferation medium was MS + 30g/L sucrose + 4g/L gellan gum;The optimal rooting medium is MS + 0.2mg/L NAA + 1.0mg/L TDZ.The combination of NAA and TDZ can not only promote root growth,but also delay plant senescence.(3)The optimal disinfection method for L.brownii is: 75% alcohol for 60 s + 2% sodium dichloroisocyanurate for 40min+1 drop of Tween 20;The optimal inducing medium was MS + 0.2mg/L NAA + 0.5mg/L TDZ;The optimal medium for proliferation and rooting is MS+30g/L sucrose +4g/L gellan gum.(4)The optimal proliferation medium for L.bakerianum is MS +0.2mg/L NAA + 0.5mg/L TDZ;The optimal rooting medium is MS +0.2mg/L NAA + 1.0mg/L TDZ.(5)The optimal proliferation medium for L.papilliferum is MS +30g/L sucrose + 4g/L gellan gum;The optimal rooting medium is MS +30g/L sucrose + 4g/L gellan gum,MS + 0.2mg/L NAA + 0.5mg/L TDZ.(6)The optimal proliferation medium for L.pumilum is MS +1.0mg/L NAA + 0.5mg/L TDZ,and the optimal rooting medium is MS+0.2mg/L NAA + 1.0mg/L TDZ.(7)The optimal proliferation and rooting medium for L.lankongense is MS + 1.0mg/L NAA + 0.5mg/L TDZ.(8)L.lophophorum is not easily induced in culture media supplemented with NAA and TDZ,and temperature factors are not taken into account.Therefore,in order to preserve this wild resource,it is necessary to establish tissue culture techniques,and related factors need further research and exploration.