Identification And Evaluation Of Drought-Resistance Of Transgenic Wheat With TaEBP Gene

As industry and agriculture develops global warming, environmental degradation and other negative factors will be the more serious, Agricultural water shortage problem has become increasingly prominent. The production situation facing the increasingly serious of wheat and other crops. Significant by improving the drought, salinity and other environmental stress tolerance to increase the crop yields, and the most economical and effective way is cultivate drought-resistant varieties to alleviate this problem. Therefore, to carry out wheat drought resistance genetic improvement research then identification and selection varieties of resistance to drought with significance to food safety.In this paper, using transgenic wheat with TaEBP gene, corresponding recurrent parent and local main variety as materials, a PEG simulated stress experiment under seed germination period and a pot experiment by control of the water under in mid and latter of growth stages were conducted, and then combined with comprehensive evaluation of subordinate function values, research of drought-resistance of transgenic wheat. Final choose to new wheat germplasm of drought resistance and water saving, agronomic traits prominent, Clarify transgenic wheat physiological and biochemical characteristics of drought under water stress, To develop wheat drought resistance research and breeding laid important basis.The main results are as follows:1.Study of drought-resistance in germination period to backcrossed three generations of transgenic wheat with TaEBP gene, the recipient parent and local cultivars.The results showed that under 20% PEG simulated drought stress conditions, all the tested varieties length of coleoptiles and plantule, quantities of root, germination rate are different degrees of decrease. The transgenic wheat less affected compared with corresponding contrast declined more slowly. One G258 length of coleoptile, G45 radicle length and quantities of root damage were minimal. Transgenic wheat are higher germination rate than control varieties under 20% PEG stress, G03, G258 germination rate were the highest.2.Study of drought-resistance in mid and latter of growth stages of transgenic wheat with TaEBP gene.Under a pot experiment by control of the water condition, The results showed that each period with the increase of water stress,all the tested materials of leaf RWC, chlorophyll content, photosynthetic rate,WUE, soluble protein content showed downtrend, the transgenic wheat declined more slowly, MDA content,plasma membrane permeability showed uptrend,but the corresponding contrast rose more steeply, The proline and ABA content of transgenic wheat can accumulate more than contrast varieties. More obvious and severe stress, in which G221 each period leaf RWC and WUE of the average the smallest decline, respectively 14.5%, 11.6%, and the smallest increase in MDA content was 25.4%; G258 proline and ABA content of the mean maximum, respectively up to5.3,5.2 times of normal water supply, while the smallest drop is soluble protein was 18.1%;G45the smallest decline of chlorophyll content was 23.8% , photosynthetic rate is less affected remained at 66.7% of the normal water, the smallest increase in membrane permeability is 1.77 times .Morphological indicators of severe stress ,G258 plant height, Spike length and space of spike leaf decline the smallest, were 11.0%, 24.1% and 7.6%, and the largest number of G258 Grain per spike, grain weight per plant the most weight, G221 plant height of jointing stage, peduncle length affected by the minimum, respectively, was 15.1%, 19.3%.Transgenic wheat for the overall performance of relatively slow response to water stress, plant height, Spike length, peduncle length, Grain number per spike and other agronomic traits are better than the control varieties, the most obvious of which G258.3.Adopted an average subordinate function values of comprehensive evaluation during germination and mid-late period of growth.The order of drought resistance G258>G45>G221 >XY22>G03>HG35>AK58>XN979>XM6.we can see that G258 has prominent agronomic traits, the better performance of drought and saving water.