Physigoical And Biochemical Characters Of Drought Resistance For Different Transgenic Wheat Strains By The W16Gene

This experiment is based on genetically modified wheat isolated plot(isolating bysurrounding trees) in YangLing modern agricultural enterprise incubation area from the year2010to2011, and its soil fertility is good,The tasted materials are Jimai19and G19-X60,G19-X61, and receptor Jimai19,Jinmai47as control. There are two kinds of waterconditions: non-irrigated farmland and irrigated farmland, which make it by controlling watercontent. Also, there are two treatments in irrigated farmland.This paper aimed at studying comparing diverse kinds of wheat under different waterstress conditions from these aspects：growth and development, physiological and biochemicalaspect, and agronomic traits. Methods of data analysis are using variance analysis andsubordinate function. What's more, to discussing the changes of physiological,biochemicalindicators and agronomic traits in genetically modified wheat W16. The main results are asfollows:1. Comparison of grain yield and yield component factors for diverse kinds of wheat underdifferent water content:Four materials in the normal water supply conditions, grain weight, grain number, acresof panicles did not reach significant difference in the level; wheat acres of panicles and grainsper spike was significantly lower than in the moderate water stress, water treatments,whilegrain weight is higher than normal water. Transgenic wheat G19,-X61and G19-X60grain weight was significantly higher than the receptor control Jinmai47and Jimai19transfer gene wheat G19,-X61, grain number was significantly higher than the receptorcontrol Jinmai47and19Jimai transgenic wheat G19,-X61and G19-X60acres of paniclesincreased, but did not reach significant difference; under severe water stress, the wheat grainnumber and acres of panicles decreased significantly, while grain weight increasedsignificantly. The production of transgenic wheat under severe water stress was significantlyhigher than the receptor species.2. Changes in physiological and biochemical indicator caused by water stress: As water stress intensifies, the relative water content, stomatal conductance,photosynthetic rate and soluble protein content showed a decreasing trend, magnitude ofdeclining is lower in genetically modified wheat G19-X60,G19-X61than in receptor controlJima19and Jinmai47. Transpiration rate decreases as water stress intensifies in all ofmaterials, but lower in genetically modified wheat than in control; intercellular CO2contentand plasma membrane permeability shows an upward trend, the magnitude of increasing ishigher in normal control than in genetically modified wheat; proline content is significantlyhigher in genetically modified wheat than in control. MDA content increased with waterstress intensified, the magnitude of increasing is higher in control than in geneticallymodified wheat.As the water stress prolongs, during grain filling stage, indicators of relative watercontent,stomatal conductance,proline content,chlorophyll content,photosynthetic rate andsoluble protein content are higher in genetically modified wheat G19-X60,G19-X61than incontrol,while indicators of relative conductance,transpiration,intercellular CO2and MDAare lower in genetically modified wheat G19-X60,G19-X61than in control.In a word, as water stress intensifies and water stress time prolongs, we can resistdrought,reduce the damage and reduce yield losses(caused by drought stress) by increasingrelative water content, stomatal conductance, proline content,chlorophyll content,photosynthetic rate and soluble protein content and decreasing relative conductance,transpiration,intercellular CO2and MDA in genetically modified wheat G19-X60,G19-X61.