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Evaluation Of Drought Resistance And Physiological Response Of Xingjiang Wild Bermudagrass Germplasm

Posted on:2017-03-12Degree:MasterType:Thesis
Country:ChinaCandidate:M M DuanFull Text:PDF
GTID:2323330488969879Subject:Grass science
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Bermudagrass [Cynodon dacytotn(L.) Pers.] is one of the warm-season turfgrass with strong drought resistance used worldwide,. Xinjiang has rich resources in wildlife bermudagrass, but its drought resistance evaluation have no systematic screening. In this study, we use 50 bermudagrass from Xinjiang in different habitats, measuring shape and physiological parameters under drought stress on seedling and bud stage, and analyzed using membership function evaluation, screening drought germplasm in order to provide a theoretical basis for breeding new varieties of bermudagrass under drought-resistant. The main findings are as follows:(1)With the drought stress increase(PEG concentration were 0%, 7.5%, 15.0%, 22.5%,30.0%). seed germination rate, germination engry, germination index decreased significantly on bud of bermudagrass germplasm(Cd016, Cd043, Cd047, Cd002, Cd034, Cd013), and indicators appear different at different PEG concentration; high concentration of PEG inhibited radicle length, plumule length and ration of radicle to plumule, seedling weight of Bermudagrass germplasm germ, and its impact was not significant at low concentrations;comprehensive evaluation considered, the optimum concentration was 15.0% PEG as a bud of bermudagrass under drought tolerance.(2) According to principal component analysis and subordinate function analysis, the seed germination and seedling growth ofbermudagrass germplasm at germinating stage were studied under 15% PEG(polyethylene glycol) stress, the drought-resistance of 50 bermudagrass germplasm from strong to weak was Cd016, Cd002, Cd047, Cd039, Cd033,Cd052, Cd037, Cd054, Cd019, Cd014, Cd038, Cd007, Cd062, Cd018, Cd057, Cd026, Cd025,Cd061, Cd009, Cd010, Cd041, Cd059, Cd028, Cd042, Cd024, Cd012, Cd029, Cd030, Cd022,Cd011, Cd015, Cd017, Cd005, Cd046, Cd031, Cd004, Cd044, Cd051, Cd006, Cd035, Cd001,Cd048, Cd036, Cd040, Cd032, Cd008, Cd021, Cd013, Cd043, Cd034.(3)Compared with the control, tiller number, chlorophyll, plant height, green leaf rate,aboveground biomass, below-ground biomass of 50 bermudagrass germplasm(in seedling stage)show a downward trend under drought stress. According to subordinate function analysis, the drought resistance of 50 bermudagrass germplasm at seeding stage from strong to weak was Cd024, Cd041, Cd022, Cd046, Cd042, Cd032, Cd014, Cd037, Cd054, Cd029,Cd025, Cd006, Cd002, Cd057, Cd047, Cd035, Cd021, Cd059, Cd036, Cd030, Cd039, Cd004,Cd013, Cd052, Cd008, Cd038, Cd034, Cd051, Cd001, Cd048, Cd011, Cd026, Cd010, Cd020,Cd023, Cd031, Cd019, Cd005, Cd043, Cd044, Cd028, Cd017, Cd012, Cd018, Cd016, Cd015,Cd033, Cd009, Cd007, Cd040.(4) With the increase of drought stress(soil water content were 70%-75%, 50%-55%,30%-35%, 10%-15%), Height, green leaf rate, aboveground biomass, belowground biomass,chlorophyll content, photochemical efficiency of 4 bermudagrass(Cd013, Cd034, Cd047,Cd016) showing an overall downward trend, Relative conductivity of leaves showed an increasing trend, Proline, soluble sugar content increased first then down trend. Compared with drought-sensitive material of Cd013 and Cd034, the decreased range of height, green leaf rate, aboveground biomass, belowground biomass, chlorophyll content, photochemical efficiency of drought material Cd047 and Cd016 was little, the increased range of leaves relative conductivity was small, and the accumulated proline content and soluble sugar content are high.(5) There are significant differences on drought resistance among different sources of Xinjiang bermudagrass materials, and there are some differences on the order of droght resistance between bud stage and seedling stage. Material Cd002, Cd037, Cd047 and Cd054 showed strong drought resistance at seedling and bud stage.
Keywords/Search Tags:Cynodon dacytotn, Identification of Drought Resistance, Bud stage, Seedling stage, Subordinate function
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