Dual-wavelength Confocal Microarray Scanner Research And Development

Biochip technique is one of most important development of science and technology since the last ten years, which has momentous strategic meaning and immense commercial opportunities. To avoid the passive status resulted from having no technical monopoly, improve the level of research and application of biochip technique in our country, research and development of the biochip scanner that holding the intellectual copyright has great significance.This project (30227002) is supported by NSFC (the national natural science foundation of China), and cooperated with Shenzhen Yishengtang Biological Products Co. Ltd. Then the phased fruits have been achieved since the project was unfolded, and approached to the level of producing the model machine of biochip scanner. It will be used to clinical application in the coming days.In this paper, biochip technique is firstly introduced as a whole and kinds of detective systems' characters in domestic and international research are compared and analyzed respectively. Thus a novel type of double-wavelength biochip scanner is put forward, which based on confocal-scanning technique and optical-mechanical two-dimensional scanning technique. And its scanning principle is also discussed and analyzed. Secondly, the experimental system-circuits are designed according to the scanning conceptual design, the circuitous philosophy and implementation of its function are focused emphatically. And scanning biochip, data acquisition and imaging are implemented by the system-circuits in experiments. On the base of the experiments, the system' s optimization is studied emphatically, and the techniques are achieved, which can be used to improve the scanning performance such as nonlinear scanning error' s calibration of f-theta lens and so on. The model machine' s design, debug and performance testing are finished, moreover the high speed scanning and data automated analysis are realized, and the performance index of 5Mm resolution and 1fluo/um2 (Cy3) etc are achieved basically when scanning by 532nm green laser. At last, the open questions are brought forward, and research are summarized and expected in this paper.