Adult Rat Bone Marrow Mesenchymal Stem Cell Plasticity In Vitro Studies,

PART ONE: MULTIPOTENT OF ADULT RAT BONE MARROW STEM CELLSCHAPTER ONE: INDUCTION OF ADULT RAT BONE MARROW STEM CELLS INTO 5-HYDROXYTRYPTAMINE-SENSITIVE NEURONS IN VITROOBJECTIVE: To identify the possibility of adult rat bone marrow stromal cells induced into phenotypic and functional neurons.MATHODS: rMSCs of passage 13-25 were grown in pre-induction media containing 10-7M all-trans-retinoic acid and 10ng/ml basic fibroblast growth factor. After the pre-induction media were removed, the cells were transferred to serum free modified neuronal induction media.RESULTS: Within 3 hrs after induction, responsive cells assumed neuronal morphological characteristics. 92.4-96.9% Neuron-specific enolase positive cells could be detected in 5 hrs. 93.9-95.2% of Neurofilament H positive cells observed after 24 h. Intracellular Ca2+ response to a ubiquitous neurotransmitter, 5-hydroxytryptamine, was monitored since both 5-hydroxytryptamine and Ca2+ are well known for their regulatory roles in neuronal proliferation, migration and differentiation. The results showed that the sensitivity of intracellularCa2+...