IL-6, Nf- B In The Expression And Significance Of TCT In Exfoliated Cells Of The Cervix

Cervical cancer is one of the most common malignancy of women, the incidence of second place in the world, second only to breast cancer. China is a country of high incidence of cervical cancer, including Shanxi Province, one of the areas with high incidence and morbidity significantly younger in recent years, the trend of a serious threat to women's health and life. High-risk human papillomavirus (HPV) infection is the major risk factors of cervical cancer, HPV infection is a chronic inflammatory process, and sustained cervical cancer HPV infection is a necessary condition, therefore, we believe that cervical cancer is cervicitis gradually evolved, it is inflammatory tumor. Current cervical cancer in many types of inflammatory aspects. Found in recent years, IL-6 is the female reproductive tract infections in the important pro-inflammatory cytokines in patients with cervical cancer body fluids, are highly expressed in cancer tissues, it is directly involved in the development and progression of cervical cancer. NF-κB is a nuclear transcription factor, exists in all cells in the body's defense response, tissue damage and stress, the process of cell differentiation and apoptosis plays an important role in information transmission. It is involved in the abnormal activation of inflammation related to many major cancers, such as gastric cancer, pancreatic cancer, cervical cancer pathogenesis, indicating progress to cancer in inflammatory mechanisms play an important role. There IL-6 NF-κB binding sites, in inflammation and tumors, IL-6 can activate NF-κB, the high activity of NF-κB and its binding sites play a pro-inflammatory or anti-inflammatory, and promote IL-6 and other inflammatory cytokine release, and promote tumor growth, proliferation, invasion and metastasis. At present, relevant organizations to study confirms IL-6 and NF-κB in their respective cervical inflammation, cervical intraepithelial lesions and cervical cancer are highly expressed, their expression rate of progression of cervical lesions with increased and cervical cancer was positively correlated with the level of which this study is to detect by immunohistochemistry in cervical exfoliated cells TCT IL-6 and NF-κB expression was observed in exfoliated cells in the TCT the expression of these two factors and histology in expression are consistent with each other while observing the expression of two factors, whether there is relevant.Objective:To compare normal, ASCUS, LSIL, HSIL cervical TCT different levels of residual samples IL-6 and NF-κB expression, and the corresponding pathological comparison, of IL-6 and NF-κB of the cytological diagnosis and histology are consistent with the two factors observed correlation between the expression.Methods:from October 2008 to December 2009 in our hospital TCT abnormal gynecological line (TBS classification> ASC) TCT specimens were 75 cases of ASCUS:26; LSIL:38; HSIL:11. Collection of TCT were normal, due to other reasons hysterectomy, pathology confirmed 10 cases of normal cervical specimens. The TCT will be made of residual paraffin-embedded cell blocκspecimens. TCT anomalies in the collection and paraffin-embedded tissue specimens of 75 patients were divided into CIN I:30 cases of inflammation; CINⅡ:22; CINⅢ:23. Immunohistochemistry was used to detect groups of IL-6, NF-κB p65 expression and expression of their mutual relations and with the corresponding histopathology were compared. Statistical analysis using SPSS 17.0 statistical software, usingχ2 test, Fisher exact test, differences between index Spearman ranκcorrelation analysis line, P<0.05 significant difference, and in pathological diagnosis as gold standard, were observed method in the diagnosis of cervical lesions consistent rate.Results:1. Using immunohistochemistry testing, the calculation of IL-6, NF-κB p65 in the cells of wax blocκ, and pathological tissue expression rate and the correlation between them, and calculate the cytology and histology coincidence between. The immunohistochemical detection of IL-6 was found positive expression rate of HSIL, LSIL, ASCUS, were normal in 81.8%,63.2%,42.3%, and 0 was progressively increased, P less than 0.05, the difference was statistically significance. NF-κB positive expression rate of HSIL, LSIL, ASCUS,63.6% of normal, respectively,47.4%,26.9%,0; was progressively increased, p less than 0.05, the difference was statistically significant.2. LSIL in IL-6 expression rate and organization of CINⅡ,Ⅲexpression difference was not statistically significant, HSIL in IL-6 expression rate and organization of CINⅡ,Ⅲexpression difference was not statistically significant, so IL-6 in the cell block HSIL, LSIL, the positive expression could reflect tissue CINⅡ,Ⅲexpression. LSIL in the NF-κB expression rate and tissue CINⅡ,Ⅲexpression difference was not statistically significant, HSIL in the NF-κB expression rate and tissue CINⅡ,Ⅲexpression difference was not statistically significant, it is NF-κB In the cell block HSIL, LSIL, the positive expression could reflect tissue CINⅡ,Ⅲexpression.3. The experimental analysis of histology in IL-6, NF-κB expression, application of analysis results of Spearman r=0.453, p<0.05, the expression of both correlation and positive correlation, consistent with its in cervical lesions The pathological changes.Conclusions:(1). TCT cells prepared piece of wax blocks and certain pathological coincidence rate can be assisted screening for cervical lesions. (2) IL-6, NF-κB expression in NILM group does not, in HSIL significantly increased expression, IL-6, NF-κB involved in the development of cervical lesions. (3). IL-6, NF-κB in the cells of wax block the expression of a certain extent, reflect the expression of the organization. (4). IL-6, NF-κB expression has some relevance, and for the positive correlation found between the pathological changes of cervical lesions.