| Objectives 1.Analysis the relationship between HPV infection and clinicopathological characteristics by detecting HPV infection in patients with cervical lesions.2.In order to provide scientific basis for the prevention,control and early diagnosis of cervical lesion patients through the expression of Nuclear associated antigen Ki67 and MTS1,multi pletumorsuppressor P16 proteins in cervical lesions.Methods 1.Exfoliated cells were collected from female patients diagnosed as cervical lesions by pathology deparment,and DNA of these samples were extracted.The DNA quality of the samples were detected by polymerase chain reaction(PCR)with β-actin primers;HPV positive rates were detected by PCR with HPV L1 universal GP5+/6+primers;High-risk HPV(HR-HPV)were detected by Real-time PCR,and HPV types with high HPV infection rate and ranked in the top three were verified by HPV E6 specific primers.2.Cytopathological diagnosis were performed for all specimens according to the 2001 Bethesda system(TBS),and the relationship between cytopathological grading of cervical lesions and high-risk HPV infection was statistically analyzed.3.The paraffin specimens of High-risk Human Papillomavirus positive were stained immunohistochemical of Ki67 and P16,and the correlations were analyzed among HR-HPV infection,the expression of Ki67 and P16 proteins with cervical lesions.Results 1.289 samples from 492 patients with cervical lesions were HPV positive.The total infection rate were 58.74%(289/492).Fifteen high-risk types were detected,including HPV16,18,31,33,35,39,45,51,52,56,58,59,66,68 and 82.Among them,the positive infection rates of HPV52(17.48%),HPV16(13.01%)and HPV58(12.60%)were the top three.2.There were significant differences in HPV infection according to ages of 492 samples,and statistical analysis revealed significant differences(P<0.05).The results of cytopathology were as follows:228 cases were Inflammation,in which 61 cases were HPV positive;70 cases were atypical squamous cells(ASC),in which 49 cases were HPV positive;93 cases were low-grade squamous intraepithelial lesion(LSIL),in which 82 cases were HPV positive;82 cases were High-grade squamous intraepithelial lesion(HSIL),in which 78 cases were HPV positive;19 cases were cervical squamous cell carcinoma(CSCC),in which 19 cases were HPV positive.HPV infection was closely related to degree of cervical lesions,the higher the degree of cervical lesions,the greater the chance of HPV infection,and there was a statistical difference(P<0.05).3.Among the 193 HR-HPV positive paraffin specimens,61 cases were chronic cervicitis(in which 7 samples were HPV52 positive,7 samples were HPV16 positive,3 samples were HPV58 positive),35 cases were LSIL(in which 7 cases wereHPV52 positive,11 cases were HPV16 positive,3 cases were HPV58 positive),78 cases were HSIL(in which 24 cases were HPV52 positive,36 cases were HPV16 positive,16 cases were HPV58 positive),and 19 cases were cervical squamous cell carcinoma(CSCC),(in which 6 cases were HPV52 positive,9 cases were HPV16 positive,and 4cases were HPV58 positive).Ki67 positive expression rates according to the order of chronic cervicitis→LSIL→HSIL→CSCC were 24.59%,60.00%,83.33%,94.74%.And the distribution of Ki67 positive cells in the squamous epithelium gradually increased in this order.P16 positive expression rates according to the order of chronic cervicitis →LSIL→HSIL→CSCC were14.75%,48.57%,84.62%,94.74%.Statistical analysis showed that there was a positive correlation between HPV52,16 and 58 infection and the banded expressions of Ki67 and P16(P<0.05).Conclusions 1.HPV total infection rate was 58.74 percent.Among them,the positive rates of HPV52,16 and 58 were higher,and ranked the top three.2.There was a significant age difference of HPV positive,and the HPV infection was positively correlated with the degree of the patient cervical lesions.3.The expression intensity and distribution pattern of Ki67 and P16 proteins were positively correlated with the degree of the patient cervical lesions,while HR-HPV infection was also positively correlated with the expression of Ki67 and P16.These results suggest that cervical lesion patients can be found in time through HR-HPV infection and expression of Ki67 and P16 were detected.Figure 24;Table 10;Reference 165. |
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