Magnolia Ephedra Decoction Of Nitric Oxide In Asthmatic Rats, Endothelin-1 And Mast Cell Degranulation,

Purpose:This study from the pathological morphology,immunohistochemistry and molecular biology,such as the perspective of Chinese medicine and western medicine Mahuangtang Honokiol Aminophylline on ovalbumin-induced bronchial asthma model in rats Winster mechanism research,and compare its position.Material and method:1.Using multi-point injection subcutaneous OVA(Ovalbumin),intraperitoneal injection of Bordetella pertussis vaccine approach rat model of bronchial asthma.2.In this experiment according to the weight check random number table were randomly divided into five groups,the normal group,model group OVA (ovalbumin),Magnolia Mahuangtang high-dose group,Honokiol Mahuangtang aminophylline group and low-dose group.3.High-and low-dose ephedra Magnolia Decoction on OVA(ovalbumin)-induced rat lung tissue morphology of bronchial asthma researchRat model by observing and analyzing the general situation,HE staining and other methods,to observe the group of pathological changes in morphology of the rat model of bronchial asthma evaluation,which studies Mahuangtang Honokiol rat model of bronchial asthma the role of mechanisms,and compare the treatment groups in the overall state and local organizations,different.4.High-and low-dose ephedra Magnolia Decoction on OVA(ovalbumin)-induced asthma in rats the expression of NO in blood researchThrough the use of nitrate reductase method to study the NO expression in rats with bronchial asthma changes in lung tissue,as well as the high-dose, low-dose Mahuangtang Honokiol the impact of its comparison,to further explore Mahuangtang Honokiol from immunohistochemistry on bronchial the impact of asthma. 5.High-and low-dose ephedra Magnolia Decoction on OVA(ovalbumin)-induced bronchial asthma in the blood of rats with ET-1 expression studiesThrough the use of radioimmunoassay to study the ET-1 expression in bronchial asthma in rats changes in lung tissue,as well as the high-dose, low-dose Mahuangtang Honokiol the impact of its comparison,to further explore Mahuangtang Honokiol from immunohistochemistry on the effects of bronchial asthma.Were observed through the microscope degranulation of mast cells further from the perspective of molecular biology to explore the treatment of Honokiol Mahuangtang mechanism of bronchial asthma.Results:1.The success of rat model of bronchial asthma;2.Honokiol Mahuangtang bronchial asthma can significantly improve the overall state of rats to reduce the bronchial and lung tissue inflammation and airway infiltration cramps,from organizational structure to reduce the extent of bronchial asthma,high-dose group than low-dose group;3.High-and low-dose Mahuangtang Honokiol may reduce the NO and ET-1 expression, and high-dose group for the excellent Mahuangtang Honokiol improve bronchial and lung tissue inflammation and airway spasm infiltration,reducing the degree of bronchial asthma,and inflammatory depth factor aspects of the pathogenesis of bronchial asthma;4.High-and low-dose Mahuangtang Honokiol were reduced rate of mast cell degranulation,effective control of the degree of allergic rats.Conclusion:1.Multi-point subcutaneous injection using ovalbumin(OVA) approach,the success of induced rat model of bronchial asthma.2.Treatment groups significantly improved the general state of rats to reduce the lung tissue of the degree of inflammatory cell infiltration,which Honokiol Mahuangtang high-and low-dose treatment group no significant difference.3.High-and low-dose ephedra Honokiol rat soup may lower blood NO,ET-1 levels and peritoneal mast cell degranulation percentage,with the effect of aminophylline close.Through the treatment process can reduce the airway and lung tissue infiltration of inflammatory cytokines,improve alveolitis, effective to alleviate airway edema,convulsions narrow.Inflammatory factors from the perspective of depth Mahuangtang Honokiol classic prescription treatment of bronchial asthma pathogenesis.