| Objection:This research is carried out with objects of Geshu(BL17,the blood meeting point),one of the eight meeting points,and rats with syndrome of cold congeal and blood stasis.It aims at comparing the effect on heomorheology caused by moxibustion to Geshu(BL17),and Xuehai(SP10), Weizhong(BL40),all of which have the similar function due to the specificity as blood cleft point,and Qiuxu(GB 40),which has no indication for the syndrome. It is also discussed about the regulation of Gehsu(BL17) on blood stasis syndrome,and provides the clinical experimental evidence for clinical treatment.Method:96 SD male rats were divided into 3 groups:control group(control groupA,B),Model group(Model groupA,B),Moxibustion group(Geshu(BL17) groupA,B;Xuehai(SP10) group A,B;Weizhong(BL40) groupA,B;Qiuxu(GB40) groupA,B). Excepting rats in control group(16),all the others(80) were kept staying in low-temperature freezer(-20℃) for 5 days of 4h each day for building model of cold congeal and blood stasis syndrome.In Moxbustion group A,the treatment was be given only once the day next to the end of 5 days model building.In moxibustion group B,the treatment was given 5 times(one per day),and 5 tips of moxa for one treatment,immediately the models building were finished.All the Point location was snipped the clothing hair off,and marked with color. The rats were confined into the cage with moxa plastered on the points(Xuehai SP10,Weizhong,BL40 and Qiuxu GB40).As soon as the rats calmed down,the moxibustion would be done.For moxibustion of Geshu(BL17),the limbs of rats should be fixed.The rats in both control group and model group should be captured without any manipulation.On the day next the end of moxibustion treatment,4 ml blood was drawn from abdominal aorta for the test of heomorheology,including blood viscosity,plasma viscosity,erythrocyte aggregation index and hematocrit.The comparison of indexes before and after treatment was done to detect the mechanism of moxibustion treatment.Result:1.Hemorheology comparison between control group and Model group:Blood viscosity:blood viscosity of model group A is higher than that of control group in each shear rate(P＜0.05);Low blood viscosity of model group B is higher than that of control group in each shear rate(P＜0.05);Moderate and high blood viscosity of model group B is higher than that of control group in each shear rate with no significant difference(P＞0.05).Plasma viscosity,erythrocyte aggregation index and hematocrit:plasma viscosity and erythrocyte aggregation index in model group A are higher than those in control group with no significant difference(P＞0.05);hematocit in model group A is much higher than that in control group(P＜0.05);plasma viscosityh and hematocrit in model Group B are higher than those in Control group with no significant difference(P＞0.05);hematocrit in model group B is obviously superior to that in Control group(P＜0.05).2.Hemorheology in Moxibustion group A:Blood viscosity:in low shear rate,blood viscosity in Geshu(BL17) group is lower than that in Model group with no significant difference(P＞0.05);in moderate and high shear rate,blood viscosity in Geshu(BL17) group are obviously lower than those in Model group(P＜0.05);in each shear rate,blood viscosity in Geshu(BL17) group is obviously lower than that in Qiuxu(GB40) group(P＜0.05).Plasma viscosity,erythrocyte aggregation index and hematocrit:all the these indexes are lower than those in model group with no significant difference (P＞0.05);plasma viscosity in Geshu(BL17) group is lower than that in Model group with no significant difference(P＞0.05);so is the erythrocyte aggregation index;hematocrit in Geshu(BL17) group is obviously lower than that in Model group(P＜0.05)3.Hemorheology in Moxibustion group BBlood viscosity:in low shear rate,blood viscosity in Geshu(BL17) group is lower than that in Model group with no significant difference(P＞0.05);blood viscosity in Geshu(BL17) group is lower than that in Qiuxu(GB40) group(P＜0.05); in moderate and high shear rate,blood viscosity in Geshu(BL17) group are lower than those in Model group with no significant difference(P＞0.05).Plasma viscosity,erythrocyte aggregation index and hematocrit:all the these indexes are lower than those in model group with no significant difference (P＞0.05);plasma viscosity and erhematocrit are lower than those in Qiuxu(GB40) group with no significant difference(P＞0.05),erythrocyte aggregation index in Geshu(BL17) group is lower than that in Qiuxu(GB40) group(P＜0.05).4.Hemorheology comparison between Moxibustion group A and B:Except blood viscosity in high shear rate and viscosity plasma,there are no significant difference of other indexes within the two groups(P＞0.05).Conclusion:1.The blood stasis is supposed to be caused by the increase of hemorheology indexes value in rates with syndrome of cold congeal and blood stasis as one of the factors.2.Geshu(BL17) is proved to have the function of improving blood circulation as the blood meeting point,due to its function of reducing hemorheology indexes values,which can provide experimental evidence for clinical treatment.3.Although moxibustion can improve blood circulation by regulation of rheology, micro-circulation and vasomotion.However,there are the differences in indexes of points in moxibustion group.The value of hemoreology indexes of Gehsu(BL17),Xuehai(SP10) and Weizhong(BL40) groups are lower than that of Qiuxu(GB40) group.Except blood viscosity and hematocrit in Qiuxu(GB40) groupA and Qiuxu(GB40) groupB,all the other indexes are superior to those in model group.All above indicate the difference of blood circulation improvement by moxbustion among the points.All above shows that Gehsu(BL17),Xuehai(SP10) and Weizhong(BL40) have indication of blood circulation improvement,the mechanism of which is to improve the blood circulation by reducing hemoreology indexes;the indication of regulating blood circulation of Qiuxu(GB40) is worse than that of Gehsu(BL17),Xuehai(SP10) and Weizhong(BL40) because that all the indexes in it are higher than those in the other 3 points.All these will provide the experimental evidence for clinical treatment. |
PDF Full Text Request