| Purpose:By moxibustion of Feishu,Ganshu acupuncture points,this paper aims to observe the distribution and allotment of calcium ion in relevant viscera after moxibustion and the rule of electric current change of relevant acupuncture points,and to study the non-linearity rule between the acupuncture points and viscera,to discuss the connotation of "one acupuncture point for multi-viscera" and provide experiment reference tbr acupuncture point selection and adjunction of clinic science.Method:Divide 40 SD white rats into 5 groups randomly in experiment 1 and experiment 3.untreated group for 10 days,Feishu group for 10 days,LPS group,Feishu LPS group and Ganshu LPS group;In addition to the aforementioned group,add untreated group for 45 days,Feishu groups for 45 days to experiment 2 with all 8 white rats in each groups. Do the moxibustion of both sides of Feishu,Ganshu acupuncture point in a row for ten days on the Feishu group for 10 days,Feishu group for 45 days,Feishu LPS group and Ganshu LPS group respectively.Pick up untreated group for 10 days,untreated group for 45 days, LPS group in similar manner without moxibustion.Materials are taken in the eleventh day from the untreated group for 10 days,Feishu group for 10 days,LPS group,Feishu LPS group and Ganshu LPS group;Materials are taken from untreated group for 45 days after 45 days of cultivation and from Feishu group for 45 days after 10 days of cultivation after Feishu moxibustion.6 hours acute inflammation prior to taking materials,inject LPS the white rat's abdominal cavity of LPS group,Feishu LPS group and Ganshu LPS group which brings about the acute inflammatory reaction of white rats,measure acupuncture point electricity and temperature once per hour since then.Blood has been taken from abdominal aorta after LPS injection for 6 hours,and lung,liver,spleen,kidney,heart organism has been taken for the calcium ion assaying.Observe the five internal organs,serum calcium ion concentration and electricity change in periphery relevant acupuncture points of the 7 post-groups after moxibustion of Feishu and Ganshu acupuncture points.Result:(1) General Situation of White Rats:There are no death of white rats during the experiment period.After moxibustion,skin moist and scar aggravates day by day in the acupuncture points of rats from Feishu group for 45 days,Feishu group for 10 days,Feishu LPS group and Ganshu LPS group,and their spirit, mobility,hair color are better than that of untreated group for 45 days,untreated group for 10 days and LPS group.After LPS injection in abdominal cavity on the rats in Feishu LPS group,Ganshu LPS group and LPS group,the rats show phenomena of activity reduction, scrunch inertia,erecting hair,color reduction,lips cyanosis,skin and tail becoming hard, abdominal respiration and lax which aggravates gradually.Therein rats in LPS group after LPS injection react more severe than that of Feishu LPS group and Ganshu LPS group.The anus temperature of the rats in LPS group and Ganshu LPS group after LPS injection on abdominal cavity is＜36℃,the anus temperature of the rats in Feishu LPS group after LPS injection on abdominal cavity after 3h is＜36℃Which implies that the white rats have already had the reaction of acute inflammatory reaction.On the present occasion,generally speaking,the other groups are better than the three groups injected LPS;and Feishu LPS group,Ganshu LPS group are better than the LPS group. (2) Electricity Change of Left Feishu Acupuncture Point:Comparison of Left Feishu Situation in the Same Group at Different Time:As compared with injection after 1h for the LPS group,the fall of current in left Feishu is more significant in 5h after injection.Current decline in Feishu LPS group after injected LPS 1h,3h,4h,and 5h.All of them,after 1h for the LPS,the fall of current in Feishu LPS group is more significant than before injection;others are the most significanted than before injection.As compared with injection after 2h for the LPS,the fall of current in Feishu LPS group is more significant in 4h and 5h after injection.Comparison of Left Feishu among Different Groups at The Same Time:Prior to LPS Injection:The electricity average values of both Feishu group and Feishu LPS group before injection is higher than other groups remarkably.The electricity of Feishu group and Feishu LPS group is higher with significant difference as compared with untreated group,Ganshu LPS group,and LPS group.2h after LPS injection:The electricity of Feishu group is higher with significant difference as compared with untreated group,Ganshu LPS group,and LPS group.3h after LPS injection:The electricity of Feishu group is higher with significant difference as compared with Ganshu LPS group.5h after LPS injection:The electricity of Feishu group is higher with significant difference as compared with LPS group.Comparison of electricity D-value of left Feishu in each time points after LPS injection minus the value before LPS injection in the same group in different time:Feishu LPS group: the negative D-value of 2h after injection and 5h after injection is higher than that of before injection with significant difference respectively.Comparison of electricity D-value of left Feishu in each comparison among groups after LPS injection minus the value before LPS injection in the different group at the same time: 4h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group 4h after injection and before injection fell dramatically as compared with untreated group and LPS group,therein the untreated group has highly significance,LPS group has significance. 5h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group 5h after injection and before injection fell dramatically as compared with untreated group, Feishu group and Ganshu LPS group,therein the untreated group has highly significant difference,others have significant difference.Electricity D-value of LPS group 5h after injection and before injection fell dramatically as compared with untreated group,which has significant difference.(3) Electricity Change of Right Feishu Acupuncture Point:Comparison of Right Feishu Situation in the Same Group at Different Time: Comparison of Right Feishu Situation in the Same Group at Different Time:As compared with injection after 1h for the LPS group and before injection,the fall of current in Right Feishu is more significant in 5h after injection.Current decline in Feishu LPS group after injected LPS 1h,2h,3h,4h,and 5h.All of them,after 2h for the LPS,the fall of current in Feishu LPS group is the most significant than before injection;others are more significant than before injection.Comparison of Right Feishu among Different Groups at The Same Time:Prior to LPS Injection:The electricity of Feishu group and Feishu LPS group is higher with significant difference as compared with untreated group,Ganshu LPS group,and LPS group,lh after LPS injection:The electricity of Feishu group is higher with significant difference as compared with untreated group.2h after LPS injection:The electricity of Feishu group is higher with more significant difference as compared with untreated group,LPS group,Feishu LPS group,and Ganshu LPS group.3h after LPS injection:The electricity of Feishu group is higher with significant difference as compared with LPS group,Feishu LPS group,and Ganshu LPS group;therein Ganshu LPS group is the most significant of them.4h after LPS injection:The electricity of Feishu group is higher with significant difference as compared with LPS group,Feishu LPS group,and Ganshu LPS group.All of them,the fall of current in LPS group has more significant difference;others have the most significanted difference.5h after LPS injection:The electricity of LPS group is lower with significant difference as compared with untreated group.This electricity of Feishu group is higher with significant difference as compared with LPS group,Feishu LPS group,and Ganshu LPS group.All of them,LPS group has more significant difference;others have the most significant difference.Comparison of electricity D-value of Right Feishu in each time points after LPS injection minus the value before LPS injection in the same group in different time:LPS group:the negative D-value of 1h after injection and 5h after injection is higher than that of before injection with significant difference respectively.Feishu LPS group:the negative D-value of 2h after injection,4h after injection,and 5h after injection is higher than that of before injection with significant difference respectively.Comparison of electricity D-value of Right Feishu in each comparison among groups after LPS injection minus the value before LPS injection in the different group at the same time: 1h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group 1h after injection and before injection fell dramatically as compared with LPS group,Feishu group and Ganshu LPS group,therein the LPS group has highly significant difference,other groups have significant difference.2h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group 2h after injection and before injection fell dramatically as compared with untreated group,Feishu group and LPS group,therein the LPS group has significant difference,other groups have highly significant difference.3h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group 3h after injection and before injection fell dramatically as compared with untreated group,LPS group and Feishu group,therein the Feishu group has significant difference,other groups have highly significant difference.4h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group 4h after injection and before injection fell dramatically as compared with untreated group,LPS group,Ganshu LPS group,and Feishu group,therein the Ganshu LPS group has significant difference,other groups have highly significant difference.5h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group 5h after injection and before injection fell dramatically as compared with untreated group,LPS group,Ganshu LPS group,and Feishu group,therein the LPS group has significant difference,other groups have highly significant difference.Electricity D-value of LPS group 5h after injection and before injection fell dramatically as compared with untreated group,which has significant difference.(4) Electricity Change of Left Ganshu Acupuncture Point:Comparison of Left Ganshu Situation in the Same Group at Different Time:LPS group: As compared with before injection,the fall of current in Left Ganshu is the most significant in 2h,3h,4h,and 5h after injection.As compared with injection after 1h for the LPS,the fall of current in Left Ganshu is more significant in 2h,3h,4h,and 5h after injection.Therein,after 2h,5h for the LPS have the most significant difference,others have more significant difference.Current decline in Feishu LPS group after injected LPS 1h,2h,3h,4h,and 5h.All of them,after 2h for the LPS,the fall of current in Feishu LPS group is more significant than before injection;others are the most significanted than before injection.Current decline in Ganshu LPS group after injected LPS 2h,3h,4h,and 5h.Therein,after 3h and 5h for the LPS, the fall of current in Ganshu LPS group is more significant than before injection;others are the most significant than before injection. Comparison of Left Ganshu among Different Groups at The Same Time:Prior to LPS Injection:The electricity of Feishu LPS group is higher with significant difference as compared with untreated group.The electricity of Ganshu LPS group is higher with more significant difference as compared with untreated group,LPS group,Feishu group,1h after LPS injection:The electricity of Ganshu LPS group and LPS group is higher with significant difference as compared with Feishu LPS group.2h after LPS injection:The electricity of LPS group,Feishu LPS group and Ganshu LPS group is lower with significant difference as compared with untreated group and Feishu LPS group,therein LPS group has the most significant difference;others have more significant t difference.5h after LPS injection:The electricity of LPS group and Feishu LPS group is lower with significant difference as compared with untreated group.Comparison of electricity D-value of Left Ganshu in each time points after LPS injection minus the value before LPS injection in the same group in different time:LPS group:the negative D-value of 2h after injection,3h after injection,and 5h after injection is higher than that of 1h after injection with significant difference respectively.Comparison of electricity D-value of Left Ganshu in each comparison among groups after LPS injection minus the value before LPS injection in the different group at the same time: 1h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group 1h after injection and before injection fell dramatically as compared with untreated group,Feishu group and LPS group,therein the untreated group has highly significance,other groups have significance.Electricity D-value of Ganshu LPS group 1h after injection and before injection fell dramatically as compared with untreated group,which has significant difference.2h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group,Ganshu LPS group,LPS group,2h after injection and before injection fell dramatically as compared with untreated group and Feishu group,them all have highly significant difference.3h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group,LPS group and Ganshu LPS group,3h after injection and before injection fell dramatically as compared with untreated group,therein the Ganshu LPS group has highly significance,other groups have significance.4h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group and Ganshu LPS group,4h after injection and before injection fell dramatically as compared with untreated group,which has highly significant difference.Electricity D-value of Feishu LPS group and Ganshu LPS group,4h after injection and before injection fell dramatically as compared with Feishu group,which has significant difference.5h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group,5h after injection and before injection fell dramatically as compared with untreated group and Feishu group, both of them have highly significant difference.Electricity D-value of Ganshu LPS group and LPS group,5h after injection and before injection fell dramatically as compared with untreated group,both of them have highly significant difference.Electricity D-value of Ganshu LPS group and LPS group,5h after injection and before injection fell dramatically as compared with Feishu group,both of them have significant difference.(5) Electricity Change of Right Ganshu Acupuncture Point:Comparison of Right Ganshu Situation in the Same Group at Different Time:As compared with injection after 2h,3h,4h and 5h,for the LPS group,the fall of current in Right Ganshu is more significant before injection.As compared with injection after 1h,2h,3h,4h and 5h,for the Feishu LPS group,the fall of current in Right Ganshu is more significant before injection.As compared with injection after 4h,for the Feishu LPS group,the fall of current in Right Ganshu is more significant after injected 2h and 3h.Comparison of Right Ganshu among Different Groups at The Same Time:1h after LPS injection:The electricity of Ganshu LPS group is higher with significant difference as compared with untreated group,Feishu LPS group and LPS group,therein LPS group has significant difference,others have highly significant difference.The electricity of Feishu group is higher with significant difference as compared with Feishu LPS group.2h after LPS injection:The electricity of Feishu group is higher with significant difference as compared with LPS group.Comparison of electricity D-value of Right Ganshu in each time points after LPS injection minus the value before LPS injection in the same group in different time:Feishu LPS group:the negative D-value of 1h after injection,2h after injection,and 3h after injection is higher than that of 4h after injection with significant difference respectively.The negative D-value of 2h after injection and 5h after injection is higher than that of before injection with significant difference respectively.Ganshu LPS group:The negative D-value of 1h after injection and 4h after injection is higher than that of before injection with significant difference respectively.Comparison of electricity D-value of Right Ganshu in each comparison among groups after LPS injection minus the value before LPS injection in the different group at the same time: 1h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group 1h after injection and before injection fell dramatically as compared with untreated group,Feishu group,LPS group and Ganshu LPS group,therein the untreated group has highly significant difference,other groups have significant difference.2h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group,2h after injection and before injection fell dramatically as compared with untreated group and Feishu group,therein the Feishu group has highly significant difference,untreated group has significant difference.Electricity D-value of Ganshu LPS group and LPS group,3h after injection and before injection fell dramatically as compared with Feishu group,both of them have significant difference.4h after LPS injection-before LPS injection:Electricity D-value of Feishu LPS group and before injection fell dramatically as compared with untreated group,Feishu group and LPS group, therein LPS group has significant difference,others have highly significant difference.5h after LPS injection-before LPS injection:Electricity D-value of,5h after injection and before injection fell dramatically as compared with untreated group,Feishu group and LPS group, therein LPS group has significant difference,others have highly significant difference.(6) Changes in each viscera and blood serum calcium ion concentration:Compared with lung,liver,heart,spleen,kidney,the blood serum Ca2+ in each same groups is higher with highly significant difference(P＜0.001),Whereas lung,liver,heart,spleen,kidney has no significant difference(P＞0.05)。Lung:the Ca2+ in lung from Feishu group for 10 days is higher with significant difference as compared with Feishu group for 45 days,untreated group for 10 days and Ganshu LPS group(P＜0.05);the Ca2+ in lung from Feishu group for 10 days is higher with highly significant difference as compared with LPS group(P＜0.01).Kidney. the Ca2+ in kidney from LPS group,Ganshu LPS group is higher with significant difference as compared with untreated group for 45 days(P＜0.05).Blood serum:the Ca2+ in serum from Feishu group for 10 days,LPS group,Feishu LPS group and Ganshu LPS group is higher with significant difference as compared with untreated group for 45 days(P＜0.05).(7) The Impact of Moxibustion of Feishu,Ganshu Acupuncture Point on White Rats' Temperature:After LPS injection on abdominal cavity of white rat,the temperature change in different time slice are shown as follow:LPS group:the temperature after injection 6h is higher than that of before injection and 5h after injection with significant difference. Compared with 2h after injection and 3h after injection,the temperature of 6h after injection is higher with highly significant difference.Feishu LPS group:compared with the temperature before injection,the temperature after 5h,6h after injection is higher with significant difference and highly significant difference respectively.Compared with the temperature after 1h,2h,3h,4h after injection,the temperature 6h after injection is higher with significant difference.Ganshu LPS group:compared with the temperature 2h after injection,the temperature after 5h,6h after injection is higher with significant difference and highly significant difference respectively.2h after LPS injection:compared with untreated group,the temperature of Ganshu LPS group is lower with significant difference.6h after LPS injection: compared with untreated group,the temperature of LPS group is higher with significant difference.Temperature difference:LPS group:the positive D-value of 6h after injection and 2h after injection is higher than that of before injection with highly significant difference respectively.The positive D-value of 3h after injection and 6h after injection is higher than that of before injection with significant difference respectively.Ganshu LPS group:the positive D-value of 2h after injection and 6h after injection is higher than that of before injection with significant difference respectively.Conclusion:1.The periphery acupuncture point electricity has itself a non-linearity dynamic variation of physiologic regularity.2.After the moxibustion of dorsovisceral points,the increase of acupuncture point bio current shows relevant acupuncture point specificity,also it has nonlinear effects of varying degrees to current in other dorsovisceral points.It implies there exists a non-linearity effect of "1 acupuncture point for multi- viscera" by stimulating acupuncture point and this nonlinear characteristic has certain rules that parts of them conform with the Allelopathy relationship of five elements theory and the rule of nearest treatment in acupuncture therapy.3.The LPS injection to abdominal cavity has caused traumata of varying degrees to channel qi of lung,liver,spleen,kidney and heart,which indicates that LPS has a non-linearity effect on each viscera.Owing to the meridian acupuncture points interconnect viscus,consequently the extent of damage caused could be known by measuring the current value of dorsovisceral points.It embodies the spirit of traditional Chinese medicine on theory of "knowing the inner from the surface","measuring and proving by the image",and "trying to figure out the inside from the outside",it also implicates that dorsovisceral points can exquisitely react the physiologic and pathological change of inner body.4.When an acupuncture point has been given a moxibustion stimulation(adjustment) and a pathologic stimulation(LPS),each viscera in body presents nonlinear effect of varying degrees under the effect of two non-linearity factors.5.The five internal organs dorsovisceral points can reflect the effect of the LPS acute injury stimulation to body more sensibly as compared with source points.6.Acupuncture point moxibustion has a positive affect on the whole condition of white rat. 2mg/kg LPS injection to abdominal cavity may bring about acute inflammation of white rat and affect the whole condition,but beforehand moxibustion can has a function of safeguarding or enhancing the white rat's resistibility to endotoxin.7.Blood serum has the most dissociating calcium ion content,there are calcium ion in the five internal organs with different content.Generally spleen has the most while the heart has the least.Whereas moxibustion effect and LPS effect can affect change of calcium ion content in blood serum and viscera.8.Feishu moxibustion has the most remarkable affect on calcium ion in lung,it can enable lung to attract calcium ion thus brings about concentration of calcium ion.And it has also a apparent effect of Ca2+ concentration to kidney and liver allelopathy in five elements theory.9.Moxibustion of Feishu acupuncture point has an apparent short-term effect on calcium ion collection but not last until 45 days.10.The calcium ion in kidney has a decreasing trend with the age increase of white rat.11.Under the effect of acute inflammation caused by LPS,moxibustion of Feishu,Ganshu can both has an effect on the collection of calcium ion in lung,whereas Feishu moxibustion has a larger effect than moxibustion of Ganshu acupuncture point.12.Moxibustion of Feishu,Ganshu acupuncture point has inhibiting effect on the reduction of calcium ion in kidney to some degree,whereas the effect of Feishu moxibustion is higher than that of Ganshu acupuncture point.13.The Feishu moxibustion has a remarkable effect on the calcium ion aggregation of lung, liver,and kidney;whereas Ganshu moxibustion has a remarkable effect on the calcium ion aggregation of liver,spleen,and lung.14.Physiologic temperature presents a regular change of dynamic equilibrium.15.LPS brings about certain traumata to body,whereas moxibustion of Feishu,Ganshu can both antagonize this affect.The effect of moxibustion on Feishu acupuncture point 1h～3h after injection is higher than that of Ganshu,whereas moxibustion on Ganshu acupuncture point reflects preferable defense effect 4～6h after injection.16.The benign effect of Ganshu acupuncture point moxibustion on liver can enable it to have a positive affect as an important organ for disinterring LPS.17.The characteristic of temperature change in each groups in each time point is similar to electricity change,its relevant rule requires further study. |
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