Aconite Decoction In Normal And Deficiency In Mice In Vivo Plasma Concentration Differences In Study

The mechanism of the Chinese Traditional medicine toxicity responds to have something to do with the property of Chinese traditional medicine, also with human body function. The human body has obvious difference to the reaction of medicine under the pHysiology appearance and the pathologic appearance, whether the toxicity medicine is fit of the dialectics usage is also an important reason to cause poison. So reasonableness understanding Chinese Traditional medicine of toxicity, investigating a Chinese Traditional medicine toxicity actively and the body relativity of the appearance, which for with Chinese Traditional medicine modern change into a chance to strengthen toxic research of the Chinese Traditional medicine and bitterly for make Chinese Traditional medicine as the natural medicine, green therapy to serve in mankind has important meaning.Aconitum carmichaeli is a kind of processed product of Aconitum carmichaeli Debx roots in the secondary root of buttercup, its functions are to cause restoration from collapse, supplement body fire and reinforce yang, dispel wind, cold and damp. The un-processed product of Aconitum carmichaili has strong toxicity; it can use when it was preprocessed to reduce toxicity. This study selects Aconitum carmichaili as the representative of poisonous Chinese Traditional medicine to study the relation between poisonous Chinese herbal medicine toxicity and body appearance.This study is a part o《fBased on the "three elements of properties constitute" real Chinese medicine research》—"973"The national research development of the point foundation plans (NO: 2007CB512605) which about the Plasma Concentration of Aconitum carmichaili, mainly by researching the difference between falsely cold appearance mice and the normal in the Plasma Concentration and pHysiology appearances, such as weight and hair color...etc., descend the influence of the different pHysiology pathologic appearance by definitely poisonous Chinese Traditional medicine and inquiry the toxicity of Chinese Traditional medicine and the body about the relativity of the appearance. In this study, the following fields of research were conducted:Firstly, While building up a HPLC examination method to analyze Aconitum carmichaili water fried liquid in aconitine, mesaconitine, hypaconitine in our laboratory, according to replication water of the clinical method making fried the liquid carry on an examination, definite among them the Content of aconitine, hypaconitine, mesaconitine is 0.112 mgs, 1.034 mgs, 0.541 mgs, which can be used to the next animal experiment;Secondly,we have built up a HPLC examination method to analyze hypaconitine in the mice serum efficiently. Making duplicate mice falsely cold appearance model, carrying on an examination to analyze hypaconitine in the mice serum in the falsely cold appearance and normal appearance mice with weight and hair color etc, is in the medicine process the variety of the pHysiology index sign to discuss the relation that body in the different appearances having different responds to the toxicity Chinese Traditional medicine .Test result enunciates that falsely cold appearance mice bear to be subjected to the toxicity which attaches Aconitum carmichaili to fry better.The mice attached Aconitum carmichaili water fried liquid after infusing the stomach to pass a survival a circumstance to see for 14 day on the 7th day , the livability of falsely cold appearance mice were obviously higher than the normally mice(2 set:The normally mice livability is 87.5%, falsely cold ppearance mice livability 100%;3 set:The normally mice livability is 70.0%, falsely cold ppearance mice livability 92.5%), also explains the mice of falsely cold appearance is stronger towards infusing Aconitum carmichaili water a fried liquid toxically of bearing and being subjected to dint.This agrees with traditional"the dialectical theory cure, prescribe medicine for specific ailment", "poison in ordinary people is poisonous, but cures illness of the patient" within theory of the Chinese medicine pHarmacology to match, proving clinical empHasize reasonable to use medicine accurately.The innovation of this experiment lies in adopting a method to compare Plasma Concentrationan between falsely cold animal model and normal animal for the very first time density, studys the correlation between medicine and body appearance, for providing a new way to study toxicity Chinese Traditional medicine.