Osteitis Pain Given The Experimental Rabbit Knee Osteoarthrosis Serum No And Sod Impact

【Objective】To clarify the mechanisms of "Gu Yan Tong Ding Fang" and provide theoratic evidences for clinical use to the osteoarthropathy of knee joint, this article uses different amount of "Gu" to treat rabbits with experimental osteoarthropathy of knee joint. Another group of rabbits with osteoarthropathy of knee joint treated by Fenbid serve as positive control.. It will check the related indexes of rabbit serum and use the immunohistochemistry method to perform pathology analysis to the cartilage of the knee joint.【Methods】Thirty healthy 4-10 months old New Zealand rabbits are divided into five groups with each group six rabbits using random and parallel control methods. They are normal saline group (A),normal saline model group (B),medium amount Chinese traditional medicine model group (C),high amount Chinese traditional medicine model group (D),and positive control group (E). The osteoarthropathy of knee joint model is built according to the Japanese KangYan standard: 0.5ml 1.6% Papain is injected to the knee joint cavity of rabbits at first, 4th and 7th day of the experiment respectively, which is performed three times continuously. Three days after the final injection, stomach perfusion is carried out: group (A) and (B) use15ml·kg^-1·d^- normal saline while group (C) and (D) use Chinese traditional medicine. After converting the amount between human and rabbit, 5.9g/kg/d Chinese traditional medicine is given to group (C) while 11.8g/kg/d Chinese traditional medicine is given to group (D). 15ml·kg^-1·d^-1 of 4mg/ml Fenbid suspension mixed with distilled water is given to group (E). 6weeks after the application of drugs, 50mlblood is cramped out by a 50ml syringe. Then the blood is treated in centrifuge to get the serum and then test the amount of NO and the activity of SOD in the serum. By the end, the animal is killed to get the cartilage and synovial membrane of knee joint.【Results】1. The movement of the knee joint of the "Gu" high amount group (D) is improved significantly and so is of the positive control group (E).2. The amount of NO in serum in group (B) is higher than the group of normal saline (A). "Gu" can decrease the level of NO in serum because the levels of NO between group (C),(D) and (B) have significant difference(P＜0.01), but the levels between group (D) and (E) have no significant difference(P＞0.05).3. The activity of SOD in group (B) decreased significantly, however, "Gu" can increase it in the serum of experimental knee joint disease rabbits. The effect in group (C) is superior than group (E) although there is no significant difference between group (C) and (D).4. "Gu" can improve the pathology performance and microcosmic structure of the experimental knee joint disease rabbits significantly.【Conclusion】"Gu" can decrease the level of NO and increase the activity of SOD in the serum of experimental osteoarthropathy of knee joint in the rabbits and the effect is superior than the positive control group. The mechanisms may concern the regulation of vasodilator factot, decreasing the injury by free oxygen, decreasing the level of hyaluronic acid in serum and increasing the expression of TGF in the cartilage and synovial membrane of the knee joint.