Differences Huang Xiaotong Of The Rabbit Knee Joint Disease Mechanisms

Objective:The osteoarthrosis is a chronic , going on disease after the middle age of pilosity , the common symptom for will exhaust oneself the joint ache, the activity will aggravate, after the rest will alleviate, the long time will not move has the stiff feeling as well as the bone is large and so on. This disease belongs to the " rheumatism " category of Chinese medicine, is a clinical common disease, its incidence of disease, the impact on patient' s life quality can't be underestimated, the impact on social productivity and financial burden produced from this are very surprising . Therefore, seeking its pathogenesis has important clinic and scientific research meaning. This experiment duplicates out the experimental rabbit' s knee bone joint disease model through imitating the joint disease of human bone, adopt different dosage of QHXTF to treat it , and with curative effect certainly Fenbid as positive to contrast group, measure rabbit serum relevant index , make to joint cartilage and slippery membrane immune group pathology cut into slices and analyse at the same time, it is should square to function mechanism of knee joint of disease , treat for side this knee bone clinic , joint of disease use medicine and offer the scientific theoretical foundation rationally to be preliminary and clear. We in line with to invigorate the kidney strong bone Chinese medicine, is it stop the pain to invigorate blood circulation, Shu Feng put through treatment principle that holds in place with a net. With QHXTF traditional Chinese medicine treat it, measure relevant index is it probe into QHXTF of the function mechanism to osteoarthrosis ,offer the experiment basis for further investigating.Methods:Adopt at random , pair blind , parallel method that contrast the old white hare of New Zealand of health divides into five groups 30 April - October, every group six, normal group (A ) , model group (B ) , hit dosage group (C ) , high dosage group (D ) , positive group (E ). Standard build mould method according to Japan Yan Kang, etc. knee bone joint inject 1. 6% of the papain protease 0. 5ml in the in rabbit, 1-4-7 , inject into rabbit of the knee joint , inject 3 times in succession one in experiment separately. Setting up bone joint disease model, irritate the stomach in 3 days after inject once at the end, A and B group irritate and take physiological saline 15ml, C and D offer the traditional Chinese medicine to irritate the stomach , the dosage group 5. 9 g *kg—1d—1 , high dosage group 11. 8g *kg-ld-l while offering after converting according to people' s rabbit separately, E group is it make with pure distilled water 4mg*ml-1 Fenbid of density mix and hang liquid 15ml to offer. Give after the six week medicine collecting blood with 50ml syringe heart to fetch animal serum . fetch after the blood putting to death animal fetch knee joint cartilage and slippery membrane. Measure the content NO in the serum ,the activation of SOD , quality of HA, utilize immune group dye and vision to analyseTGF-β lexpress the intensity in joint cartilage and slippery membrane .Results: 1. QHXTF dosage group can improve rabbit knee joint function activity , knee joint of disease notably to disappear, Fenbid group too can more obvious improvement rabbit knee bone knee joint function activity .2. Model group content NO of serum , higher than normal group , QHXTF can reduce level NO of serum , in the traditional Chinese medicine, high dosage and positive group and model group have significance difference (P<0. 01 ), in the traditional Chinese medicine, high dosage group and positive group do not have obvious difference (P> 0.05 ).3. Model group activation SOD of serum , reduce notably, QHXTF can improve serum SOD activation, traditional Chinese medicine dosage group function superior to positive group (P<0. 05), dosage group and high dosage group for raise rabbit serum hit activation of SOD have signify-cance difference .4. Model group content HA of serum , higher than normal group notably (P<0. 01), QHXTF can reduce level HA of serum notably...