Sika Deer Velvet Extraction And Activity Of The Collagen

Object Study on the extraction separation, properties and activity of the collagen extracted from the deer antler.Method The collagen was extracted using alcohol from deer antler. The methods, such as alcohol-extraction, BCA^TM protein assay, RE-HPLC, SDS-PAGE, acid hydrolysis and SEM was used. We did the active test to the collagen. Osteoporosis rat models were established by ovariectomy. To observe the level of BMD, calcium in the bone, serum BGP, AKP, NO, NOS; and the tissue of bone .To count the result by excel program.Result We have receive the results are that the collagen type I is the major component of the thing extracted form the Deer Antler using alcohol, and the extraction ratio of the collagen is about 10%, compare to the fresh deer antler. The molecular weight of the collagen is distributed in 100 kDa～200 kDa and up 200 kDa. The tests showed that collagen have stimulant effects on the growth of rat osteoblast. The collagen can improve the bone quality of the osteoporosis rat.Conclusion The collagen extracted from deer antler was collagen type I .The rate of the extraction and the purity of the collagen are very high. The tests showed that collagen have stimulant effects on the growth of rat osteoblast. The collagen can improve the bone quality of the osteoporosis rat.