Oplopanax Ginseng Active Ingredient

Oplopanax elatus Nakai is the dry root, rootstock and stem of Oplopanax elatus Nakai(bge.)Baill, it is a rare medicinal material especially in Changbai Mountains of China. According to reports, its efficacy is similar to Ginseng; it can be used to cure neurasthenic, hypotensive, cardiopathy, sugar diabetes, rheumatism and so on. In recent years, reports show Oplopanax elatus Nakai is consisted of saponins, flavonoids, coumarins, proteins, glucides, phenols, organic acids, tannins, volatile, and grease, but the study on the active components in the Oplopanax elatus Nakai are not comprehensive, especially on protein, that have not bereported by now, and study on pharmacology is very poor. According to the previous research,we did much study on the protein, and we obtained a protein from Oplopanax elatus using gelfiltration chromatography of Sephacryl S-100 and ion-exchange of DEAE Sepharose Fastlow, which was named OENPI. Its molecular weight is 26.65496 KD. Its pharmacology of first step showed antibacterial effect. In the chemical component aspect, I have got four compounds from Oplopanax elatus by using macroporous resin chromatography, silica gel column chromatography and so on. They were identified by ESI-MS, ¹HNMR, ¹³CNMR,ESI-MS, DEPT, H¹-H¹ COSY, which were determined as sucrose, syringoside, rhein,β-sitosterol. On volatility components aspect, I extracted by supercritical installation ,which was determined composition and quantity identifying by GC/MS. Protein and rhein are discovered firstly in this plant.