| The study assessed the pharmacologicalaction of the 60% ethanolic extract of stem bark of O. hurridus(OH) in the treatment of psoriasis. The animal model of tail scales of mouse was taken. To be divided into three groups: the normal saline negative control group, the low dose group, the high dose group. The effect of OH on epidermal differentiation was detected by the samples of mouse tail. The results showed that two groups of OH possessed significantly promoting effect on the formation of granular layer in mouse tail scale epidermis(p＜0.01) and there were for 4 to 5 granular cell layers and bigger granular cells in scales under microscope. The research indicated that the possible mechanism against psoriasis of OH was to romote the formation of granular cells.Furthermore, by means of silica gel column, conventional ODS column and PHPLC, 6 compouds including 2 new compouds were isolated from root and stem of O. elatus Nakai and stem bark of O. hurridus in all and their structures were elucidated on the basis of spectroscopic data and chemical methods. The 2 new compounds are oplopanoside A and oplopanoside B respectively.Meantime, syringenin was used as reference substance to establish HPLC fingerprint of stem bark of O. hurridus. Separations and determinations were carried out by linear gradient elution under following conditions: Kromasil C18 column(250mm×4.6mm, 5μm), pre-column Zorbax(12.5mm×4.6mm,5μm), acetonitrile -0.05%phosphoric acid solution as mobile phase, detection length of UV at 220nm, flow-rate of 1.0ml/min, column temperature of 35℃. HPLC system was applied to obtain the chromatograms of 11 samples of OH stem bark, 1 sample of OH root bark and 1 sample of OE stem bark. Then similarity calculations and clustering analysis were applied for data analysis. The results showed HPLC fingerprint could be used for identifying the origin of these two species. |
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