Caspase-3 And Laminin B And Study On Relationship Between Recurrent Spontaneous Abortion

Objective To investigate the effect of blockade of costimulatory signal CD86 at the time of implantation on the expression of Caspase-3 and Laminin B proteins at the maternal-fetal interface and pregnant outcome in murine abortion-prone model,which would provide some theory evidence for clinical therapy to URSA.Methods Female pregnant CBA/J mice of Experimental group and Control experimental group were received an i.p injection of 100μg of anti-mouse CD86 mAb in 200μl PBS or irrelevant-isotype matched ratIgG2b on day 4.5 of gestation,respectively.As for the normal pregnant group,nothing was received.Pregnant CBA/J mice were killed on day 13.5 of gestation.Then,calculated the embryo resorption rate,and immunohistochemical method was employed to detect the expression of Caspase-3 and Laminin B proteins.Results(1)The embryo resorption rate in experimental group was significantly reduced in comparison with that in control experimental group(χ~2=7.441,P＜0.05).(2)The positive expression rate of Caspase-3 in experimental group was significantly reduced in comparison with that in control experimental group(P＜0.05).The positive expression rate of Laminin B in experimental group was significantly increased in comparison with that in control experimental group (P＜0.05).Conclusions Blockade of costimulatory signal CD86 at the early stage of gestation can induce Caspase-3 and Laminin B to exert immunologic tolerance by their different mechanisms and reduce the embryo resorption rate of the abortion-prone model to that of normal pregnant level.