The Studies Of The Relation Between Transcription Factor Foxp3 And Embryo Damage

Objective: To observe the expression of Foxp3 mRNA and protein in human endometrium of non-pregnanct(NNP) and decidua of normal pregnant women(NP) and embryo damage gravidas.Methods: Expression and distribution of Foxp3 in proliferative phase endometrium(14cases),secretory phase endometrium(11cases) and decidua of 30cases of NP women and 27cases of embryo damage gravidas were obsverved by immunohistochemistry;The percentage of CD4+CD25+ Tr in deciduas and peripheral blood from 18embryo damage gravidas,23 normal non-pregnanct women(NNP),33 normal early preananct women(NP) were detected by double-labled flow cytometric analysis. Expression of Foxp3 mRNA and protein in deciduas of NP women and embryo damage gravidas was detected respectively by semi-quantitative RT-PCR, Real-time PCR and Western Blot.Results: (1)By immunohis- tochemistry,The expression of Foxp3 mRNA were observed to be localized in the cytoplasm in both secretory endometrium and pregnancy decidua,but not in proliferative endometrium.Foxp3 expression in embryo damage gravidas were lower significantly compared to NP women(p<0.01).The expression of Foxp3 in secretory endometrium were lower significantly than that in NP women(p<0.05);(2)Expression of Foxp3 mRNA in decidus of embryo damage gravidas was significantly lower compared to NP women by semi-quantitative RT-PCR and Real-time PCR(p<0.05).(3)Expression of Foxp3 protein in deciduas of embryo damage gravidas were significantly lower than that of NP women by Western Blot analysis(p<0.05).Conclusions:These results suggested that the expression of Foxp3 in secretory endometrium and decidua would be an important factor during the implantation and related with the maintenance of pregnancy.The lower expression of Foxp3 in embryo damage gravidas involved in the pathogensis of embryo damage gravidas.