Study Of Nine Iris Chemical Composition

Jiujie Changpu belongs to genus Ranunculaceae plants of Anemone altaica Fisch.exe C.A.Mey.. It can remove obstruction, soothe the nerves, eliminate dampness, stimulate the appetite, detoxicate and expell worms. Because its function is similar to Acorus gramineus Soland, these two traditional Chinese medicine are always confused. But it is indicated by experiment that they are very different in plant configuration, microstructure and chemical ingredients. In order to searching more constituents and establishing integrated quality standard of Anemone altaica Fisch, the author carried out the following studies.The author have a preliminary systematic test first by basic chemical methods, detect and analysis all kinds of constituents in Anemone altaica Fisch. Then extract and separate five compounds by the way of chromatography, including silica gel column and preparative thin layer chromatography and reversed-phase column chromatography etc. These five compounds structural elucidation was performed by spectroscopic analyses, primarily NMR, ESI-MS. Five compounds are established asβ-sitosterol (Ⅰ), Succinic acid (Ⅱ), Anemonin (Ⅲ), Succinic anhydride (Ⅳ), Oleanolic acid (Ⅴ). CompoundⅣandⅤare isolated from the plant for the first time. At the same time, we get 25 flavonnoids and 17 fatty acid by GC-MS,of which 23 flavonnoids and 17 fatty acid are isolated from the plant for the first time.The study lays chemical foundation for the medical curative effect research of the monomeric constituent of Anemone altaica Fisch.exe C.A.Mey., the study establish the foundation for the quality standard improvement and the medical curative effect constituents exploration of Anemone altaica Fisch.exe C.A.Mey.. In addition, the study gives out the scientific basis to differentiating Anemone altaica Fisch and Acorus gramineus Soland.