Preliminary Study Of The Chemical Constituents And Biological Activity Of The Two Tip

"LiangTouJian" is the rhizome of Anemone raddeana Regel (Ranunculaceae), iscommonly used in Chinese folk medicine for curing rheumatism and neuralgia.In thisthesis, the investigation of chemical constituents and bioactivities of "LiangTouJian"arereported. The main research results are stated as below:1.After deely studying of the chemical constituents of "LiangTouJian", 24 compounds wereisolated.(All of them were elucidated on the basis of physico-chemical method, spectralanalysis and by comparison with some authentic samples. Among these compounds, two arenew compounds, They were elucidated as:raddeanosideR₁₉(3-O-α-L-rhamnopyranosyl-(1→4)—-β-D-glucopyranosyl-(1→4)-α-L-arabinopranosyl-oleanolicacid28-O-β-D-glucopy-ranosyl-(1→6)-β-D-glucopyranoside(14);raddeanosideR₂₀(3-O-α-L-rhamnopyanosyl-(1→2)-[β-D-gluco pyranosyl-(1→6)-O-β-D-gluco pyranosyl-(1→4)]-α-L-arabinopranosyl)-oleanolic acid 28-O-β-D-glucopy ranoside(15). Other 22 known compounds are LinoleicAcid(1),3-acetyloleamolic acid(2), lupeol(3), betulin(4), betulic acid(5), diosgenin(6),oleanolic acid(7),β-sitosterol(8)s, oleanolic acid3-O-α-L-arabinopyranoside(9), eleutherosideK(oleanolic acid3-O-α-L→rhamnopyranosyl-(1→2)-a-L-arabino pyranoside.eleutheroside K 10), Oleanolic acid-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosl-(1→4)]-α-L-arabinopranoside(11),Oleanoli cacid -3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranosl-(1→2)-α-L-arabinopranoside (12), hederasaponinB(3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopy-ranosyl(1→4)-β-D-glucopyra nosyl(1→6)-β-D-glucopyra noside 13),hederacholichis E(3-O-α-L-rhamno pyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]ide-α-L-arabinopy- ranosyloleanolic acid 28-O-α-L-rhalnnopyranosyl(1→4) -β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside 16),raddean oside R₁₆ (3-O-α-L-arabinopyrnosyl(1→3)-α- L-rhamno-pyranosyl(1→2)[β-D-glucop yranosyl(1→4)]-α-L-arabinopyrnosyl oleanolic acid 28-O-α-L-rhamnop yranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside 17), raddeanosideR₁₇((3-O-β-D-glucopyranosyl(1→4)-β-D-glucopyranosyl(1→3)-α-L-rhamnopy-ranosyl(1→2)-α-L-arabinopyrnosyl oleanolic acid 28-O-α-L- rhamnopyranosyl (1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside 18), raddeanoside R₁₈(3-O-β-D-glu copyranosyl(1→4) [β-D-glucopyranosyl(1→2)]-α-L-arabinopyrnosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D- glucopyranoside 19),Leonloside D(3-O-β-D-glucopyranoside-(1→4)-a-L-arabino pyranosyl 27-hydroxyoleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D- glucopyranosyl(1→6)-β-D-glucopyranoside 20), hederacholichisideF(3-O-β-D-glucoy ranosy(1→4)-[α-L→rhamnopyranosyl -(1→2)]-α-L- arabinopyranosyl27-hydroxyolean olicacid 28-O-α-L-rhamno pyranosyl(1→4)-β-D-glucpyran osyl(1→6)-β-D-gluco pylrano-side 21), raddeanoside R₉(3-O-α-L-rhamnpy ranosyl-(1→2)-β-D-glucopyranosyl- (1→2)-α-L-arabinopranosyl 27-hydroxyoleanolic acid 28-O-α-L-rhamnopyranosyl (1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside 22),raddeanosideR₈(3-O-α-L- rhanm opyranosyl-(1→2)-β-D-glucopyranosyl- (1→2)-α-L-arab inopranosyl-oleanolic acid 28-O-α-L- rhamnopyranosyl(1→4)-β-D- glucopyranosyl (1→6)-β-D-glucopyranoside 23),evonymitol (24).2.Analyzed by GC-MS, the results of the chemical constituents of the fatty oil fromAnemone raddeana Regel showed that fifty compounds were isolated and identified,accounting for 87.5% of the total fatty oil. And analyzed by GC-MS, The results of thechemical constituents of the oil compounds from column chromatography showed thatthirty-two compounds were isolated and identified.3..Bioactivities were deeply studied on the extraction and pure compounds from"LiangToujian".(1)Anti-tumor activitya.We preliminarily screened the total raddeanoside of "Liangtoujian" to S₁₈₀ andHepAH.The results showed that the inhibitory rate of tumor of total raddeanoside wassignificant and but low.b.Comparison of the proliferous function to four kinds of cancers cell stub on refinedTRNS and pure compoundsThe results was that the extraction with three sugars or less(compound 11-13,sigle sugarchain with 3-aglycone) had more effective anti-tumor activity in vitro than those with moresugars(compound 14-16,double sugar chains with 3,28-aglycone)The inhibitory rate of tumor of refined TRNS was lower than that of TRNS. RefinedTRNS was attained after TRNS was decloured by D941 resin and enriched by D101 which made small polar compounds were lost. Further, the results showed that big polar compoundswith saponin of more sugers might go with lower or noneffective inhibitory rate of tumor in"Liangtoujian".(2) Anti-inflammation activity.We preliminarily screened the different extraction of Liangtoujian".The results were that theBu-OH and water extraction had anti-inflammation activity.4.Study of the pharmacognosy about"LiangToujian"Plant morphology, resource distribution and characters of "LiangToujian" werestudied.