| The paper consists two parts: literature review and experimental study. First Section:Literature SummaryThere are two literature reviews. The first article is review on the selection and application of animal model in the studies on the spinal cord injury (SCI) of late years at home and abroad. It contains four major portions: the choice of the experimental animal, the choice of the damage segment, the choice of the SCI model, the latter management of SCI rats. The second article introduces the clinical and experimental survey of the acupuncture and moxibustion on the SCI in rats.Second Section: Experimental ResearchObjective: To discuss the protective mechanism of electroacupuncture (EA) in the acute spinal cord injury.Methods: Rats were divided into five groups stochasticly: Blank Group, Sham Operated Group, Model Group, Drug Group and EA Group. The model rats were made a spinal cord contusion by using a modified Allen's method.(1) Comprehensive evaluation of neural function of SCI rats by CBS .(2) Detect the calcium ion content in serum and injury local tissues after injury by MTB method.(3) The injured tissues of EA Group,Blank Group and Model Group were removed.Total RNA was extracted, mRNA was purified and marked by Cy3 or Cy5 fluorescence labeling to synthesizec DNA hybridized probe that was hybridized with 27K Rat Genome Array chip. Then the chip was scanned with Lux Scan 10KA scanning apparatus. The data were analyzed by Gene Pix Pro 4.0 software system. Analysis the function of the differentially expressed genes by bioinformatics technique.(4) Detected the gene expression condition of the Capn8,Sphk1 and Ccl2 by qRT-PCR..Results: Six hours after the SCI,(1) Compared with the model group, the score of CBS level in EA Group and Drug group were significant differently(P<0.01).(2) Compared with the Sham Operated Group, the calcium ion content in serum and injury local tissues were increased in Model Group, EA Group and Drug Group. The calcium ion content in serum and injury local tissues of EA Group and the Drug Group decreased significantly as compared with those in Model Group (P<0.05).(3)There were 266 differentially expressed genes in Model Group, involved in multifarious functions, such as cytotoxicity, neural tissue degeneration and necrosis, inflammation after injury, the loss of the rehabilitation and regeneration ability, and so on. There were 181 differentially expressed genes in EA Group, 51 genes were not differentially expressed in Model Group, 36 up, 15 down.Concerning functions, such as cell metabolism, ionic channel homeostasis, immunity and defense, cytoskeleton protein, extra cell ularmatrix, cell development,etc.(4) the Capn8,Sphk1 and Ccl2 gene expression was up-regulate in Model Group and EA Group. This result is in good agreement with the gene chip experiment.Conclusion: EA had protective effect on the ASCI through depressing the calcium ion content. There were lots of differentially expressed genes in SCI rats. The rise of the Capn8,Sphk1 and Ccl2 gene expression level may be correlated with the ascension of calcium ion. EA can regulate the changes of gene expression induced by SCI. |
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