| Objective To explore the diagnostic value of blinded protected-specimen brush technique in the pathogenic diagnosis of candida infections of pulmonary. Methods A prospective, self-controlled clinical trial design, enrolled 30 patients with tracheotomy was conducted during a 8-month period. Mean age of the patients is 66.0±11.8 ,All cases clinical suspicion of candida infections of pulmonary without previous antifungal agent, using protected brush from contamination ,aspirating species from the lower respiratory samples were cultured. Meanwhile looking directly at the brushing slide samples , autopsy confirmation were performed . To investigate the culture significance between blinded protected-specimen brush technique and routine sterile aspirate tube, and histological confirmation of lung candida infections .Results 30 cases with tracheotomy, using blinded protected-specimen brush technique, species from lower respiratory samples suspected candida infections were cultured , the positive rate is 63.33%. Compared with routine sterile aspirate tube two techniques show no statistical significance, P>0.05. The positive rate of histological confirmation was 36.67%. Compared tracheotomy cases to non-tracheotomy patients, the results of using blinded protected-specimen brush technique are less contamination, P<0.05. Conclusions Receiving inadequate use of broad-spectrum antibiotics and lower values of serum albumin and neutrophil leucocyte are relatively common among 30 cases. It might be linked to a higher incidence of candida infections. Blinded protected-specimen brush technique can be used as a safe and an effective method to diagnosis the candida infections of pulmonary. The PSB may be more specific than sensitive. |
