Antisense Vegf In Prostate Cancer Cells In Experimental Research

Tumor cells release soluble angiogenic factors inducing neovascularization, a process referred to as the "angiogenic switch". At present, many research focus on tumor angiogenesis, and anti-angiogenesis is becoming a reasonable and great potential valuable new way in tumor therapy.VEGF was first described and is most important angiogenetic factor. It was found that inhibition of VEGF angiogenesis can decrease tumor neovascularization and accelerate apoptosis. Angiogenesis is also a critical requirement for local proliferation and metastasis in prostate cancer. It was reported by foreign research that VEGF had an elevated expression in advanced prostate cancer, and after the role of VEGF was blocked in many ways, it resulted in inhibiting prostate cancer growth, metastasis and inducing its apoptosis, But reviewing literatures from wanfang data, CJFD and CBMdisc in 10 years, no correlated study was reported.Hence, in this study we firstly evaluated the expression of VEGF in LNCaP, and its derivatives LNCaP C42, which exhibits a metastatic phenotype by Western blot. It was found that there existed the expression of VEGF in both cell lines, but VEGF production by LNCaP C42 was significantly higher than LNCaP. It was demonstrated that the expression of VEGF increased followed up prostate cancer invasion and metastasis. This implies that VEGF may play some role in prostate cancer invasion and metastasis. Furthermore, anti-sense VEGF was constructed and transfected into prostate cancer C42. The effect of antisense VEGF was studied in tumor cells biological features such as, proliferation and invasion in vivo and in vitro. C42 proliferation was inhibited by antisense VEGF in vitro. The cells of antisense VEGF were injected subcutaneously into nude mice in vivo. Compare to control, it was found that tumor cells transfected with antisense VEGF growth slowly, the tumor sizes minished and angiogenesis apparently decreased. It was demonstrated that inhibition of VEGF can inhibit tumor growth and angiogenesis ?It was suggested that inhibition of VEGF is a valuable therapeutic way in prostate cancer.