The Liver Labor Experimental Study On Mechanism Ii

Object: To discuss the reason of GanLao occurrence based on the experiment that analyzing parasympathetic nerve-receptor-second messenger and pathologic histology changes of iris sphincter muscle when accommodation continues.Methods:Tree Shrew was chosen as experiment object and was made staring continuously.After 3.6,9 days respectively,their iris were separate to quantitate the MAChR,cAMP,cGMP and to stydy pathologic histology changes.Results:(1)The iris sphincter muscle of Tree Shrew is more like human's thanouse and rabbit. (2)After 3 days of staring,the MAChR in the iris sphincter muscle of Tree Shrew wasn't change distinctly; After 6 days of staring,the MAChR was hypersensitivity; After 9 days of staring,the MAChR was up-regulated.(3) After 6 days of staring,the cGMP in the iris sphincter muscle of Tree Shrew increased, cAMP in the iris sphincter muscle of Tree Shrew wasn't change distinctly;(4) After 6 days of staring,the collagen and the a -SMA in the iris sphincter muscle of Tree Shrew increased.Conclusion:With the results of the former study, the persistent erethism of parasympathetic and the disorder of the balance of sympathetic and sarasympathetic double innervation controlled iris sphincter muscle and ciliary muscle may be one of important reasons of GanLao occurrence.