| ObjectiveWe established the rats in diabetic myocardiopathy model. Observing the effect of the Su-Jiang-Tang(S JT) on the blood glucose , blood pressure, body weight, index of left ventricle (left ventricle weight/body weight), blood TG and T.chol, ultrastructure of myocardial cells, expression of TGFP| and AT1 in myocardial tissue in diabetic rats induced by injecting STZ into abdomen. This experiment was to investigate the mechanism of the protective effect on the early diabetic myocardiopathy of SJT and provide an experimental basis for further investigating and clinical use.MethodThe diabetic rats were induced by injecting Streptozotocin(STZ) into their abdomen successfully, and they were stochastic divided into five groups: diabetic model group "model group"(12 rats), low dosage of SJT group "low dosage group" (10 rats), high dosage of SJT group "high dose group"(10 rats). Metformin group "drug I group" (10 rats), Metformin and Losartan group "drug II group" (10 rats); the other 10 normal rats were the normal control group "normal group". The respective dosage of drugs was given to the treated groups and the same dose of sodium chloride injection was given to the normal and model series by stomach rearing once per day for eight weeks. After therapy, the body weight, the blood glucose, blood pressure, index of left ventricle , blood TG and TCH, ultrastructure of myocardial cell, expression of TGFP| and AT| in myocardial tissue of each group were tested. All the results were analyzed by SPSS 11.0 for windows.Result1 Body weight There were no significant differences among all groups before treatment ( P>0.05 ). After treatment, the body weight of the high dosage group was distinctly heavier than that of the model group, drug I group, dug II group respectively ( P < 0.01 or P < 0.05 ) . And the effect of the low dosage group, drug I group, drug II group had no obvious difference before and after treatment.2 Blood glucose There were no significantly differences among the blood glucose of all the treatment and model groups. The blood glucose of every treatment group was more lower than that of the model group(P < 0.05 ) . The blood glucose of the high dosage group was more lower than that of the drug I group, drug II group respectively (P < 0.01). The effect had obvious different among all the treating group before and after treatment ( P < 0.05 ) .3 TG and T.CH The TG and T.CH of every treatment group was more lower than that of the model group( P < 0.05 ). There were no obvious difference among the low dosage group, the high dosage , the drug I and the drug II group.4 Blood pressure The blood pressure of the high dosage group and drug II group was more lower than that of the model group (P < 0.05 ) .5 Index of left ventricle Compared with the model series, the high dosage and drug II group could markedly decrease the Index of left ventricle(P < 0.05). There were not distinct difference between the effect of the high dosage and drug II group.6 the expression of TGFB1 and AT1 Compared with the model series, SJT could obviously reduce the expression of TGFpi and AT1 in the myocardial. The best group were the high dosage group and drug II group.7 Van-Gieson's staining The VG's staining showed that the model group rats' myocardial tissue collagen turned obviously (show the red), each other series could prevent myocardial tissue collagen from turning.8 Ultrastructure The observation of myocardial ultrastructure showed myoarchi-tectonic derangement of typical myocardial myocomma, wided parazone, widened space between intercalated disks, myofibrillar degeneration fragmentation and vacuolization, mitochondrial compensatory proliferation swelling shortened crista and rhegma of membrane, lipid droplets and glycogen granule could be seen in the cytoplasm of cardiac myocytes in the model series. The high dosage and drug II group could markedly protect ultrastructure of myocardial cells.ConclusionSJT certainly has prot... |
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