Arterial Infusion Chemotherapy On Cervical Cancer Dna Content, Cell Cycle And Apoptosis

Objective:To study the effect of intra-arterial infusion chemotherapy on DNA content, cell cycle phase and apoptosis in cervical carcinoma.Methods:Using flow cytometry, we determined DNA content and S-phase fraction(SPF) of the normal cervical tissues and the cervical carcinoma tissues before and after intra-arterial infusion chemotherapy, which involved 20 cases respectively, DNA content was decided by DNA index (DI). In order to study the apoptosis of cervical tissues, apoptosis index (A]I) was detected by DNA in situ terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick ending labeling(TUNEL).Results:1. The DI values were significantly higher in both of cervical carcinoma tissues before and after intra-arterial infusion chemotherapy (1.31?.34,1.10?.15) than that of normal cervix tissues (1.01±0.06) (P<0.01,P<0.05) .The DI value of cervial carcinoma tissues after intra-arterial infusion chemotherapy was significantly lower than that before intra-arterial infusion chemotherapy (P < 0. 05), and there were no significant difference between stageⅡand stageⅢ, Ⅳ in cervical carcinoma group(P> 0.05). 2.There were 13 cases having DNA aneuploidy before intra-arterial infusion chemotherapy and 6 cases having DNA aneuploidy after intra-arterial infusion chemotherapy and the difference was significant (P < 0. 05). 3. S-phase fraction (SPF) of cervical carinoma before intra-arterial infusion chemotherapy (24.93±9.46%) and after3intra-arterial infusion chemotherapy (14. 03±6. 59%) were significantly higher than that of normal cervix (6.99±4.07%, P <0.001).The SPF of cervical carcinoma after intra-arterial infusion chemotherapy was significantly lower than that before intra-arterial infusion chemotherapy (P<0. 001). The SPF of stageⅢ, Ⅳ was significantly higher than stagell(P<0. 05).4. The SPF of DNA asneuploidy (29.09±9.67%) was significantly higher than that of DNA diploidy (18. 51±4. 31 %, P<0.01) .5.The Al values of cervical carinoma tissues before and after intra-arterial infusion chemotherapy (5. 2 ±1. 19%, 10. 1±3. 6%) were significantly higher than of normal cervix tissues(2.5±0.95%, P<0. 001). The Al value of cervial carcinoma tissues after intra-arterial infusion chemotherapy was significantly higher than that before intra-arterial infusion chemotherapy(P <0. 001).Conclusions:1. Intra-arterial infusion chemotherapy can inhibit the reproduction of DNA in cervical carcinoma and the progression of neoplasm. 2. Intra-arterial infusion chemotherapy can reduce the SPF of cervical carcinoma tissues and inhibit cell proliferation. 3. Interventional chemotherapy can induce apoptosis of human cervical cancer cells.