Flos Sophora Anti-oxidation And Tissue Culture Method For The Study Of The Medicinal Ingredients Of Sophora Japonica

The thesis mainly studies the antioxidation of sophora. japonica and theway how to obtain the flavonoids of sophora. japonica by means of tissueculture method.The study is devided into two stages:In the first stage, 60 female mice were randomly devided into fivegroups: Normal control group; Model control group; Large-dosage group;Small-dosage group; Ginseng group。 Sophora. japonica(300mg. kg^-1. d^-1, 200mg. kg^-1. d^-1) is fed to Large-dosage group andsmall-dosage group for 20 days. ginseng (4g. kg^-1. d^-1) is fed to ginsenggroup for 20 days. Distilled water is fed to control groups for 20 days. (Theaverage body weight of mouse is 25g). All the mice are kept from food for24 hours, except for Normal control group. Next, all the mice except forNormal control group mice are injected ALLOXAN (150mg/kg) intoabdominal cavity. After three days, all the mice are killed. Hb, liver index,liver glycogen and SOD, MDA in blood and liver are measured.The results show:(1) In oxygen Model, sophora, japonica can lower obviously abnormalactivity of SOD in blood and liver. In blood and liver, sophora. japonica canlower the oncentration of MDA that had produced due to impair.(2) Sophora. japonica can also inhibit the abnormal increase of Hb.sophora. japonica can maintain the normal level of liver glycogen; sophora.Japonica can also maintain the normal level of liver index.All the results exhibit that flavonoids of sophora, japonica can eliminatesignificantly free radical and improve antioxidative ability of body, anddecrease the damage of free radical to the body. Flavonoids of sophora.Japonica has anti-aging effect and preserving liver effect. retard senility.They also exhibit that flavonoids of sophora. japonica can promoteendurance capacity level to a degree.The purpose of latter stage is to enlarge source of sophora. japonicaflavonoids.First, the flavonoids contents are derived from sophora. japonica,pseudoacacia and Robina hispida respectively. According to the result,sophora. japonica is chosen as raw and processed materials of tissue culture.Secondly,The sophora. japonica is devided into four parts:stamen(Y)'petal (B)' style (ZH ) 1 sepal (T ). They were cultured respectively on MSmedium supplemented with 2. 4--D and 6--BA. In order to obtain the better'tissue culture factor, callus induction,callus incresing amount rate and there1ative content of flavonoids in callus are determined respectively.The results indicate:(1 )To the petal (B)callus, 2' 4--D0. 05mg. L--1, 6 --BA0. 5mg. L--l or2.4--D0. 05mg. L--l, 6--BA1mg. L--1 or 2.4--D0. 2mg. L--1, 6--BAlmg. L--lis all chosen as the better hormone concentration of tissue culture.(2)To the 8tamen (Y)callus, 2' 4--D0. 05mg. L--1, 6 --BA0. 5mg. L--1or 2. 4 -- D0. 5mg. L--l, 6 -- BA0. 5mg. L--lis all chosen as the betterhormone concentration of tissue culture.(3)To the style (ZH)callus, 2. 4 -- D0. 05mg. L--1, 6 --BA0. 5mg. L--lor 2. 4 --D2mg. L--1, 6--BA1mg. L--1is all chosen as the better hormoneconcentration of tissue culture.(4)To the sepal (T)callus, 2. 4--D0. 05mg. L--l, 6--BA0. 5mg. L--l or2. 4 --D0. 2mg. L--1, 6 --BA1mg. L--1is al1 chosen as the better hormoneconcentration of tissue culture.At 1ast, in order to improve the concentration of flavonoids in callus andrealize the commercial valPe, the paper studies precursor(Phe,Tyr)how toaffect the concentration of flavonoids in tissue culture.The results stiggest:In the process of tissue culture, the Medium supplemented with Tyrproduces better results than that supplemented with Phe.