| Heating is one of the most important means of TCM processing, the degree of the heating processing has a decisive influence on the medicinal properties and the effect variation scope of traditional medicine. The traditional method of judging heating degree of has been so subjective and arbitrary obviously, not objective, parameterized and standardized, that it’s difficult to ensure the heating degree uniformity, which means it’s difficult to ensure the stable quality of processed products. In order to study the effects of different heating degree on the chemical composition of traditional Chinese medicine, mathematics representation, meanwhile, is introduced to stand for the heating degree, so to carry out parameterization, objectification and standardization on heating degree, finally to ensure uniform heating degree under the condition of scientific controllable heating, process controllable and quality stable, further to ensure the safety and efficacy of TCM clinical application. The mathematical model Characterizing the processing temperature was established and sophora japonica acted as the model drug experiments, provided a new thinking and new method to study the scientific connotation of heating degree and processing mechanism.The main researches were as the following:1. Establishment of the mathematical model of the processing heating degree. Established the mathematical model of the processing heating degree for single component and multi-component.according to the principle of chemical dynamics.2. Effect of heating degree on the quality of the sophora flower bud processed by different heating degree:Following Chinese pharmacopoeia and the relevant literature, the chemical components of the crude and the49kinds of heat-processed product of the sophora flower, which included water, total ash, acid insoluble ash, extract, flavonoids, tannin, rutin and quercetin. were determined. The author found the content of all these chemical components in the sophora flower bud changed after processing. The water content significantly decreased with the increase of heating degree, while the total ash and the acid insoluble ash content increased with that, but the rest chemical components, having the same variation tendency:at first, it climbed up with the increase of heating degree and then, it declined with that when the heating degree went to its climax.3. Study on the mathematical model of the sophora flower bud. The established mathematical model of the processing heating degree was applied to pilot research of the single component such as rutin, quercetin, flavonoids and tannin, and the multi-component (the total of rutin and quercetin) extracted from the sophora flower bud, and the reliability of these mathematical model were proved by calculation. 4. Study on the HPLC fingerprint of the sophora flower bud processed by the different heating degree:In order to study the influence of different heating degree on the quality of the sophora flower bud further, this paper set up the HPLC fingerprint with15common peaks of [he sophora flower bud water decoction, Chromatographic conditions included Ultimate XB-C18column(4.6mm×250mm,0.45μn), the measurement wavelength was256μm, the column temperature was35℃, the mobile phase consisting of a mixture of methanol-0.4%phosphoric acid by gradient elution. The flow rate was1.0mL/min, and the sample size was20μL. And the similarity and the clustering analysis were conducted in regard to the fingerprints of the different products processed by the different heating degree. The author found, in the same series of processed product, the heavier the heating degree, the less similarity between the processed products and the crude one. The similarity between the processed products under close heating degree was larger than not. No.H1-1, the processed product under the minimum heating degree, had the maximum similarity with the crude, which indicated that the light healing degree a fleets its chemical composition slightly. On the contrary, the No. H7-7, the processed product under maximum healing degree, had the minimum similarity with the crude, which indicated that its chemical composition changed visibly after carbonizing by stir-frying with the maximum heating degree. It can be seen from the cluster analysis results combining the relative retention time and the relative peak area of the common peaks of the sophora flower bud processed by the different heating degree, that the difference of the relative retention time between the processed products by different heating degree was minor, but the difference of the relative peak area was relatively major. The50kinds of processed products can be divided into two classes, the processed products by heavier heating degree, such as No. H7-6and117-7, belong to the first kind, and the rest fit into the second kind. In the second kind, the processed products like No.113-7, H7-5and H6-7were close relatively. And the processed products by heavier heating degree, such as No. H6-6, H3-6, H2-7and H5-7, were close to the crude in the relative peak area of the common peak. It indicated that the changes of the relative peak area of the common peak of the processed products by heavier heating degree were listed from significant to unobvious within a certain range. And it demonstrated that the content of these chemical components in processed products would tend to the crude one when increased or reduced to a certain quantity during heating process.5. Study on the effect of kinetic model of the components extracted from the processed products of the sophora flower bud by different heating degree. Three kinds of processed products, including the crude, the medium heat product (H4-4) and the maximum heat product(H7-7), were extracted dynamically. The effects of kinetic model of the rutin and the quercetin extracted from the3kinds of processed products were studied. The author found it was significant that the parameters of the kinetic model of the different components extracted from the processed products by different heating degree was varied. This research can provide a certain of experimental and scientific basis to explore the parameters of extracting process of the same Chinese medicine processed by different heating degree. |
