Disease And Syndrome Rat Model Of Endometriosis And Evaluation

Endometriosis (EMT) refers to the endometrial tissue growth function in uterine cavity coating mucosa appeared outside of other parts of the body's disease. Is a kind of hormone dependence, hemorrhagic, inflammatory, hereditary disease, is one of the common gynecologic, healing abilities, and the recurrence rate is higher, and the difference in recent years incidence increased dramatically. The incidence of a disease in women of child-bearing age as high as 10%～15%, accounting for the common gynecologic surgery, and 30% above gradually increasing, is clinically women dysmenorrhea, infertility, the main cause of chronic pelvic pain.Many physicians to uterus the pathogenesis and treatment methods of researching, think endometriosis clinical pain and discomfort, serious impact on the women's physical and mental health, give women of child-bearing age of life and work to bring a great deal of pain. Its, though benign disease, but symptoms stubborn, have extremely easy relapse, again hair characteristics, ectopic endometrium of organization can like malignant tumors grow and transfer as spread, without ideal treatment, efficacy are limited and temporary. The latest statistics, any method of treatment, and the recurrence rate in 5 years above 40%, treatment is tricky. Therefore, will endometriosis listed as one of the complicated diseases.Traditional Chinese medicine (TCM) without "endometriosis" concept. But according to the clinical manifestations of the disease of TCM "dysmenorrhea," "remove lump" and "no son" and "menstruation" category. The causes for TCM called the "blood", from the when they do not, when diarrhea, stagnant body diarrhoea don't become yu blood. "Stasis" is to generate endometriosis series the main cause of symptoms and signs. Stasis resistance cell palace, cell veins, cell winding impassability, general clauses pain; Stasis resistance blunt as to infertility or menstruation; Stasis sluggish day long, accumulation and become remove lump; Blood and qi-blood stagnation, become born clinical many syndrome.Although in recent years the EMT's study of penetrated, but its pathogenesis is still not quite clear, the treatment effect is not ideal. According to recent research shows that Endometriosis patients exist hemorheology of change and immune disorders. The occurrence of this disease is more cytokine joint participation, the result of joint action. The change of blood rheology and cells and humoral immune function abnormalities, cause endometrial cells adhesion, planting, invasive, of the formation of new blood vessels, eventually leading to the endometrial tissue in the peritoneal cavity planting, hyperplasia. Some scholars find endometriosis patients within the abdominal cavity liquid macrophages active strengthening, planting lining of the organization can produce a series of cell factors and growth factors, these factors through secreted oneself and paracrine mechanism and macrophages has close interaction between of ectopic endometrium relationship in the planting and growing peritoneum, and has an important function of the reproductive process produces cytotoxic effects. Due to ethics and other reasons, it is difficult to observe different disease patients, in the whole process of different disease onset of human, not comparing research, also cannot injury study. Therefore, establishing the ideal human animal model to simulate the EMT, repeated experiments, controlled in animals has cause pathology, medication, research, can conduce to conquer disease as soon as possible.This topic through the "disease" and "card" of the method of combining, establish and blood stasis EMT rat model. The first through surgery method of copy endometriosis rat model, then according to TCM theory, using drugs, gentle disposition stimulate many factors such as composite method, causing further qi stagnancy and blood stasis syndrome, and through the comprehensive evaluation methods validation syndrome, and the objective existence for disease and syndrome animal models combined with new methods for establishing the EMT, traditional Chinese medicine research provides the reliable tool.Objective1, to explore the complex factors in the role of autologous traplants EMT model of rats was established, and blood stasis caused by the EMT rat model method.2, through the screening of pathogenic factors, animal behavior, performance of observation, objective indicators testing methods, and drugs for animal model proves to evaluate, summarized a set of scientific and objective evaluation system.Methods1, autologous transplants after EMT model of rats was established, and the factors causing further the stimulation method and blood stasis syndrome, and to establish the qi stagnancy and blood stasis endometriosis models. 2, through optimizing made mould factors; Observe the external signs and each animal behavioral performance; About group animal histopathological and laboratory testing; index Drugs such as animal model proves step four established model evaluation system.Results1, through the data analysis, qi stagnancy and blood stasis EMT model of rats macro and micro as follows:(1) the macro characterization: rats showed eye conjunctival vessels obviously are dilated, auricle lip weeks dark red, claw armour tail dark purple, tongue rusted, etc.; And the activity decreases, outlining the crouching, coat, osteoporosis, irritability, is not easy to catch. And blood stasis complies with the macroscopical performance.(2) hemorheology: disease and syndrome combined with model group compared with pure disease model group, whole blood viscosity of high cut, cut, cut the whole blood viscosity, lower reduction, cutting, high cutting red, fibrinogen rigid index were significantly increased, both more statistically significant (P<0.05). Disease and syndrome combination group compared with blank of whole blood viscosity, XieChen low shear XieChen equation, were all significantly higher K value, the two more statistically significant (P<0.05). And simple disease model group, compared with blank group whole blood viscosity, high cutting in the whole blood viscosity reduction of cutting, high cutting, red blood cells were significantly reduce the rigid index, both more statistically significant (P<0.05).(3) monoamine neural transmitter: disease and syndrome combination group serum DA, NE content in disease model group is high, there is a significant difference (P<0.05). But blank group of serum DA, NE content is higher than disease model group, disease and syndrome combined group, and partially group were significant difference (P<0.05).5-HT each group there were no obvious difference.(4) ET, NO:ET content in disease and syndrome combined group higher than the disease model group, both model group was significant difference (P<0.05). Disease model group ET/NO higher than the blank group, has significant difference (P<0.05). And disease and syndrome combination group than disease model group, blank group NO content is high, there is a significant difference (P<0.05).2, drug animal model analysis proves This lab, frutus Que group compared with disease and syndrome group with significantly reduced plasma viscosity, there were significant differences between the two group (P<0.01), but fibrinogen increased significantly, there were significant differences between the two group (P<0.01); Four jun group compared with disease and syndrome of ESR reduced, with two more statistically significant (P<0.05). Whole blood viscosity of high, medium and low cut, whole blood viscosity reduction of high, medium and low cut, erythrocyte deformation, rigidity, electrophoresis index, there were no obvious difference. Four jun group serum ET significantly lower levels of disease and syndrome, both with significant difference (P<0.01); NO, ET/NO value has no obvious differences between groups. DA, NE,5-HT content in each group no obvious differenceConclusion1, abdominal transplantation rat model of established EMT, with high survival rate, easy to observe, celiac adhesion properties, such as general transplantation less survival rate for 78%. By pelvic microenvironment and abdominal wall effect of transplant tissue growth environment, and the pathogenesis of endometriosis environment more similar, is more suitable to evaluate clinical research.2, using multi-factor exciting method to establish qi stagnancy and blood stasis, the EMT model of rats hemorheology many index, serum monoamine neural transmitter DA, NE content were significantly higher in pure EMT disease model group, shrinking vascular material ET content were significantly higher in pure EMT disease model group, a statistically significant (P<0.05). From the macro and micro indications on performance and blood stasis are in line with the performance, this model has the characteristics of reliability and repeatability, for the EMT clinical and experimental research provides a good carrier. But the blank group of serum DA, NE content is disease group, disease and syndrome combination group are high, in order to prevent blank rats, single cage of depression, we raised produce control group 5～6 only every cage breeding, the phenomenon needs to be further study.3, this lab, frutus Que group, four jun group compared with disease and syndrome model group in serum ET,four jun group was obviously lower than the combined group, disease and syndrome significant difference (P<0.01). After surgery may for rats damage blood, after fill gas treatment but changed its blood circulation state, and synephrine Que group do qi and activate blood circulation after treatment, NO, ET, ET/NO group compared with disease and syndrome with NO obvious difference. Each group monoamine neural transmitter is no obvious difference. It said the drugs in this experiment proves successful, also need not model have been studied.