Gouty Arthritis Damp And Hot Card With Disease And Syndromeanimal Model Research

Objective:Through the many factors made of composite model method, with the wide application of hot and humid card in the rat model and classic gouty arthritis model as a comparison, this paper explores the establishing of traditional Chinese medicine gouty arthritis damp and hot card in the rat model method, aims to develop gouty arthritis damp and hot card disease and syndrome with animal model research, for disease and syndrome with combined therapy of the clinical curative effect of the mechanism research provides the new ideas and experimental basis.Methods:1. A diet high in fat and sugar diet:ordinary feed and use honey water free drink, and press the big rat body quality, service and the grease filling liquor alternate.2. The group choose healthy adult SD rats only40, according to random method will rats can be divided into4groups:blank control group (group A), gout group control group (group B), hot and humid groups in control group (group C), gout and hot and humid group (D group), each group of10only.3. Made mold in high fat and sugar diet, hot and humid environment comprehensive factors, building a damp and hot card gouty arthritis animal model:(1) the normal control group:in constant temperature and humidity environment, distilled water free drink, ordinary feed;(2) the gout groups:normal feed+distilled water free water+articular cavity injection drug;(3) hot and humid group:high fat and sugar diet+hot and humid environment;(4) the gout and hot and humid group:high fat and sugar diet+hot and humid environment+articular cavity injection drug.4. Observation of the indexes and the detection observed experimental rat made before and after a general condition of mould, food and drink, and quantity, water quantity, weight, defecate properties of change. With Bradford method to detect the water rat urine protein channel2(AQP2), radiation immune method to detect endothelin (ET) and somatostat (CGRP). Rats after death, quickly remove ankle synovial organization, tongue organization, in10%of formaldehyde solution fixed, paraffin slice made, HE dyed, light-microscopy.Results:Endothelin (ET), somatostat (CGRP), AQP2damp and hot card as the quantitative indexes, the blood uric acid, and gait, joint organization morphology change as the index of gouty arthritis. A group of each index for normal (comparing with), B group compared gait appear to change, joint synovial organization morphological changes, in group C endothelin levels, somatostat reduce, urine AQP2content increases, testing results and the group D B and C two groups of comparisons if at the same time appear B, C two group of change (blood, gait, joint organization uric acid morphology, urine AQP2content), suggests the model making success.Conclusion:The results show that, in the high fat and sugar diet+hot and humid environment+articular cavity injection drug under the comprehensive function, can be copied successfully gouty arthritis of damp and hot card animal model. This method is to "disease and syndrome combination" gouty arthritis of damp and hot card animal models of the experimental study of active exploration, and will be of disease and syndrome with combined therapy of the clinical curative effect of the mechanism research provides the new ideas and experimental basis.