Expression And Significance Of Nkcc1 In Axillary Sweat Glands Of Palmar Hyprhidrosis Patients With Axillary Hyperhidrosis

Objective Testing palmar hyperhidrosis with armpit sweat hand sweat patients, has no sweat armpit sweat hand and non-axillary sweat glands of patients sweat glands number and Na+K+Cl-cotransporter isoform (NKCCl) expression, Explored the relationship between the incidence of palmar hyperhidrosis.Methods 27 cases of palmar hyperhidrosis with axillary hyperhidrosis patients (axillary hyperhidrosis group), 11 cases of palmar hyperhidrosis with no axillary hyperhidrosis patients (simple type of palmar hyperhidrosis group),8 cases of non-palmar hyperhidrosis patients (control group),take each group of patients with axillary line of paraffin section of skin to make hematoxylin-eosin staining, counting the number of sweat glands,and adopts freezing microtome, using immunofluorescence technic to detect NKCCl in the three groups of patients with axillary sweat glands in the skin.Results All three groups of sweat gland counting was not statistically significant(HC=2.528, P=0.238). NKCCl immunofluorescence technict experimental part. The average optical density of NKCCl,The axillary hyperhidrosis group were 1.2657±0.11651,the simple type of palmar hyperhidrosis group were 1.1659±0.17140,the control group were 1.1117±0.12465. Image analysis and statistics show that axillary hyperhidrosis group and the simple type of palmar hyperhidrosis group and the control group was statistically significant differences between the average optical density (F= 5.158, P= 0.041; F= 5.158, P= 0.006), simple type of palmar hyperhidrosis group and the control group no statistical significance (F= 5.158, P= 0.384).Conclusion Palmar hyperhidrosis with armpit sweat hand sweat disease patients sweat increase in association with NKCCl protein expression increased, but not with the number of axillary sweat glands has nothing to do.