Expression And Significance Of AQP5 In Axillary Sweat Glands Of Palmar Hyperhidrosis Patients With Axillary Hyperhidrosis

Objective: Examine existence of aquaporin(AQP) in axillary sweat glands of palmar hyperhidrosis patients with axillary hyperhidrosis and investigate its relationship with the pathogenesis of palmar hyperhidrosis patients with axillary hyperhidrosis.Methods Axillary sweat glands were harvested in 34 cases of palmar hyperhidrosis patients with axillary hyperhidrosis ,including 32 of high grade,2 of middle grede,,according to Lai standard,and 8 cases of non—hyperhidrosis as control group.The number of axillary sweat glands was statisticed by using HE staining and the expression of aquaporins(AQP1,AQP3 AQP5) was detected by using immunochemical method(SP method) respectively. The comparison was made between hyperhidrosis group and control group.Result The result of HE staining shows the sweat gland include the secretion part and vessel part;the karyon of sweat gland cell show itself blue,the cytoplasm red;and the sweat glands of some people get together showing conglomeration,the sweat glands of some people single in optic microscope.The number of axillary sweat glands in two groups haven't the distinction in statistics(Z=-1.506, P=0.132). The result of Immunohistochemistry shows both groups have expression of AQP5,no expression of AQP1,AQP3; The AQP5 expression of axillary sweat glands in hyperhidmsis cases was significantly increased as compared with that in control group(Z=-3.523, P﹤0.001).Condusion the number of axillary sweat glands of both groups haven't the distinction in statistics.AQP1 and AQP3 may not involves secretion of human axillary sweat glands.AQP5 has the relationship with the axillary hyperhidrosis of palmar hyperhidrosis patients with axillary hyperhidrosis.; The increasing of AQP5 is probably one of pathogenic mechanisms for palmar hyperhidrosis patients with axillary hyperhidrosis...