Expression And Clinical Significance Of Akt2 In Colon Carcinoma

Objective To investigate the express of serine/threonine kinases 2 (AKT2) in colon carcinoma and normal colon tissue and the role of the PI3K/AKT signal pathway in the occurrence and development of colon carcinoma. To analyze its corre-lation with clinicopathological features of colon carcinoma.Materials and Methods Immunohistochemistry(IHC) and immunofluorescence (IF) were used to determine the expressive levels of AKT2 protein in specimens of colon carcinoma and normal colon tissue in the surgical margin collected from 65 patients. Then the results and the clinical features were statistically analyzed.Result The positive expression of AKT2 in colon cancer tissue is 69.23% while that of the surgical margin tissue is 6.15%.The AKT2 protein expression level in colon carcinoma was significantly higher than that of the surgical margin(P<0.05).There are correlations between AKT2 expressive level and colon carcinoma invasiveness, lymphathic metastasis, pathological grading as well as Dukes stage respectively(P<0.05),but no correlation was found between AKT2 expressive level and gender, age, tumor differentiation and histological type(P>0.05).Conclusion AKT2 play an important role in the development of colon carcinoma.AKT2 can regulate its downstream target genes by the PI3K and AKT sig-naling pathway. The PI3K and AKT signaling pathway is crucial for tumor cell grow-th, angiogenesis, genome instability and tumor cell invasion and migration. Further-more, AKT2 can improve cell tolerance to hypoxia and was associated with chemore-sistance. The AKT2 protein over-expression in colon carcinoma play an important role in pathological grading, metastasis and prognosis. For this reason, AKT2 has become a viable drug target for colon carcinoma therapy and provided a new target for gene therapy.