Expression And Significance Of Iron Ion, Mda, Dmt1 Around Hematoma In A Rat Model Of Intracerebral Hemorrhage And Neuroprotective Effects Of Taurine

OBJECTIVESIntracerebral hemorrhage (ICH) is one of the most common and devastating types of stroke. It occurs when a blood vessel ruptures or there is vascular anomaly, flooding the surrounding tissue with tissue. It accounts for 35% of all strokes in Asian countries, but responsible for approximately one half of all stroke-related deaths. Despite many efforts directed at clarifying the roles of surgical and medical therapy for ICH, the most appropriate management is still controversial. Incidence rate of ICH increases as the society prospers and people grow older. The mechanism of the delayed brain damage after intracerebral hemorrhage (ICH) is still complicated and not well understood.So it is signify-cant to study on mechanism of iron toxicity process and intervention in ICH. There are few data about DMT1 functions on iron overload after ICH. There are also few reports about the topics abroad and domestic data now. This topic is aimed at exploring the dynamic state change of Iron ion,MDA,DMT1 around hematoma, and the possible connections between them in a rat model of intracerebral hemorrhage and neuroprotective effects of Taurine.METHODS54 rats are randomly divided into sham-operated group (n=18) and intracerebral hemorrhage group (n=18) and intracerebral hemorrhage treatment group (n=18).The sham-operated group, intracerebral hemorrhage group and intracerebral hemorrhage treatment group are divided into 3 subgroups respect-ively at 3 hours,3 days and 7 days after the establishment of animal models, every subgroup including 6 rats.Intracerebral hemorrhage and intracere-bral hemorrhage treatment group are induced by stereotaxic injection of autologous blood into the right caudate nucleus of the rats (normal saline is injected in sham-operated group). Intracerebral hemorrhage treatment group are given Taurine 300mg/kg/d by ip qd after the establishment of animal models. Cerebral hemorrhage models are duplicated with the method created by Yang to imitate the natural state of clinical hemorrhage. H-E staining and the light microscope are adopted to observe morphology changes of Iron ion and nerve cells. Elisa is used to detect the expression of MDA and DMT1 around cerebral hemorrhage regions. SPSS13.0 software is used to carry out statistical analysis. The differences of groups are analyzed by t-tests and ANOVA.RESULTS1 A large number of iron ions appear in 3 days and reach the peak in 7 days. Compared with those of the blood group and the sham-operated group, the relative areas of iron ions in treatment group decrease statistically significantly.2 MDA begins to rise from 3 hours, and increases significantly from 3 days to 7 days. MDA of treatment group decreases significantly either in 3 days or in 7 days. There is positive correlation between the fe2+ and MDA through the correlation analysis.3 DMT1 increases in brain hemorrhage group than in sham-operated group and decreases in treatment group. There is positive correlation between the fe2+ and DMT1 through the correlation analysis. But there is no statistical significance between d3 and d7 group either in treatment group or in intrace-rebral hemorrhage group.CONCLUSION1.Iron ions may play an important role in the mechanism of the delayed cerebral edema after cerebral hemorrhage. The abnormal accumulation of iron ions participates in the injury of brain tissue surrounding brain parenchyma after ICH. Iron overloading resulting from ICH is possibly an important cause of the delayed brain insults.2.Iron overload induces DMT1 expression after ICH. DMT1 may play an important role in iron handling in ICH.3. The increase of MDA indicates that the iron has relations to the free radicals in cerebral hemorrhage.4.In the rat model, taurine treatment can lower the Iron ion levels. The conclusion is the same as one topic from the magazine of Circulation. Reduced cardiac iron levels may be related to the tonic inhibition of L-type Ca2+ channels by taurine.5.Taurine has an insignificant effect on DMT1.6. Taurine can make a significant reduction in MDA. It has a protective effect on the brain tissue surrounding hematoma. Taurine is a natural sulfur-containing molecule of oxygen free radical scavenger, suggesting that it can reduce the damage of free radicals on the structure and activity of SOD. Indicate us that the taurine medical preparations can be made to speed up the recovery effect of hemorrhagic stroke.