| Object The mechanisms involved in brain edema formation following intracerebral hemorrhage (ICH) are very complex . Evidences have indicated that the red blood cell lysis within the hematoma is one of important processes responsibled for brain edema formation . And iron which released from hemoglobin due in part to brain injury after ICH. Therefore , we examined how about the alteration of iron during brain edema formation after ICH and whether such iron will be cleared by the iron chelator.Methods Male Sprague-Dawley rats received an infusion of 100μl autologous blood or needle placement only into the right basal ganglia. These rats were intraperitoneal infused the iron chelator-- Desferal and salt solution respectively 24h or 72h after operation and were performed the behavioral test 1, 2 ,3 ,7, 14 or 21 days later after being given drugs , then killed. The number of rats in every time point was 6 (n=6) . One part were determined the brain water and ion contents. And brain nonheme iron content was determined with another part.Results Perihematomal edema peaked around the third day or fourth day after ICH , remained for more than a week, then declined gradually within 2 weeks. Brain nonheme iron began to ascend in the third day , peaking about 2 weeks later , a marked increase was not cleared within 3 weeks. The intraperitoneal infusion of iron chelator-- Desferal significantly improved the neurology dysfunction of rats throughthe decreased scores of behavioral test and brain edema was also attenuated . Furthermore , Desferal blocked the process of nueronal death due to the iron toxicity, and alleviated brain injury with the effects on reducing the brain iron content.Conclusions Brain edema after ICH is due to the release of iron from hemoglobin. The iron overload caused cellular injury and neurol deficits during ICH. Desferal could reducing the oxidative stress caused by the release of iron from the hematoma, so Desferal may be a potential agent for ICH therapy. |
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