Quality Standards Of The Wild Poppy

The alkaloids is one of the important components in Papaver nudicaule L., such as amrensinine, re-framidine, nudicauline and nudicaulonol and so on, Nudicauline is found to be a good anodyne and will not cause addiction. On the development of the study on Papaver nudicaule L., these alkaloids in Papaver nudicaule L. have been shown, for example: anodyne, anti-chronic diarrhea and so on. It's very important for studying and exploiting the alkaloids in Papaver nudicaule L.. To fulfill the mission of the People'Republilc of China PHarmacopoeia, that is to say the determination of the chemical of nudicauline in Papaver nudicaule L. will be added. this thesis includes four parts as follows:Review. A review of the alkaloids in Papaver nudicaule L. and the quality standard of Chinese traditional medicine were given.Experimental study1, Studies on the quality standard for Papaver nudicaule L..A method for determination of liquiritin in Papaver nudicaule L. by HPLC was established.2, Study on the fingerprint of the nudicauline in Papaver nudicaule L.. A method for the fingerprint of the nudicauline in Papaver nudicaule L. by HPLC was srudied. 3, Compare morpHology, microscopic strucrure and HPLCchromatograms of Papaver nudicaule L. with Baishan Papavernudicaule. The comparison research on morpHology, microscopic strucrure and HPLC chromatograms for Papaver nudicaule L .and Baishan Pavernudicale is beneficial for discrimination of the two drugs. The result will provide foundation for the research on pHarmacology of the two drugs, and will promote exploitation and utilization of the two drugs.Result and discussion The mobile pHase was consisted of methanol-water-ethylamine(550:450:0.45) .and the detection wavelength was at 256nm. Then the content of nudicauline in ten samples was determined by this method. The result showed the content of nudicauline was between 0.001%～0. 008965%, the linear correlation was good (r=0.9996) and the average recovery was 96.5%. This method was simple reproducible and rapid, which was suitable for determination of nudicauline in Papaver nudicaule L..The original plant identification, macroscopical identification, microscopical identification and pHysical and chemical identification between Papaver nudicaule L. and Baishan Papavernudicaule were observed.