| Tar DISTareUTION AND CRANGE OF ELECTROACUPUNCTU'RE ON TIIEIFNry LIus IMMUNOREAETIVE PRODUTION IN RAT BRAINANSTAnCTThe exPression of IFN- y like immunoreactivity(IFN- Y-li) in rat brain was stUdied and thecorrelation betWeen acuPunCtUre and immuno-neuro-endocrine net was discussed. The rat weregrouped to contrOl group, Zusanli POint grouP, immunosuppresive group and immunosupPtessive+electroacupuntUre grouP. The ZuSanli point of rat was electrOacupuntUre by using acupunctUreinstrUment (G6805-2A, intensity was 3V frequent was 2HZ, time was 30 minute), and the changes ofthe distribUtion of IFN- Y -li in cerebral corteX, hippocaInPus, ePendyIn4 hyPOthalamus, CerebeIlum,medullny viscetal zone were stodied by using the technique of inununohistOchemiStry SR The resultSas fOllow;:l. Cerebral cortexThe IFN- Y -li neurons were abundanly distributed in all layer of cerebra cortex, but especiallywere distributed in the lnyers of III. V. Compare with control group, The number of IPN- Y -lineurons in electroacuPwte at Zusanli point had no significan difference. The IFN- Y -li neuronshad significant difference in lagers of llI. V, when compared wun immunosuPpressive grouP. It isrecreasing in lagers of I 1 II' W' V,bul there is not significant in statiStics. The resultS showed thatIFN- Y existCd in cerebral cortCx of tat, and IFN- y may play the role of immunomodulation in cerebralcortex.2. HipPOcampusThe lFN- Y -li positive neurons extensively distributed in CAl' CA2. CA3 of hippocampus incontrOl group, especially in CAl, and more positive neurons were in the pyramidal cell layers of thehippocampus .In few positive neurons waS deteCted in CA2. CA3. The effect of EA on IFN- Y -lipositive productS in hippocampus wasn't obvious when compared with control group. The IPN- Y -lipositive cells were extensively detected in CAl of hippocampus when compared withimmunosuppressive group.The resultS suggested that the IFN- y may play the role ofimmunomodulation in hippocampus.3. EpendymaThe IFN- Y -li POsitive cells were distribUted in LV V3, Aq, V4. ComPared with cchl grouP, thereis no signican in electrOacupunbe group. The change Of IFN- Y -like positive cells were not obviousin Zusanli group when comPared with control group. The Iru- Y -li POsihve cells in AqcimmunosuPpressivatEA grOuP much than immunosuPPressive grOup. The resultS suggsted that the IFN-y can Synthnhe or absorb in ependyIna and the immunsuPPressive +EA grouP can lead tO occur IFN-?IFN- y may be a novel neuopeptide in epen dyma and modulate brain-CSF circuit.4. HypothalamusThe IFN- i -ii positive cell were detected in nucleus paraventricularis, nucleus periventricularis, nucleus supra-opticus, nucleus suprachiasmaticus, nucleus mamillaris posterior Compared with control group, electroacupunture group bad no signicant difference in the number of the IFN- Y -Ii positive cells in hypothalanus. The effect of inimunosuppressive+EA group on IFN- y -Ii positive cells were obvious in nucleus paraventricularis, nucleus periventricularis when compared with cimmunosuppressive group. The result showed that the hypothalamus have inmunomodulation and the immunosuppressive+EA group can lead to occur IFN- Y.5. CerebellumThe IFN- 慳?positive fibers were widely distributed in stratum moleculare of cerebellum , The IFN慳?-ii positive cell were weekly occasionally detected in those. The IFN- 慳?-li positive cell were widely detected in layers of Purkinje and weeklyin the stratum graulosum. Electroacupunture group had no signicant difference in the number of the IFN- 慳?-ii positive cells when compared with control group.The IFN- 慳 -Ii positive cells were obvious in stratum Purkinje, when compared with cimmunosuppressive group. The result suggested that IFN- 慳?could have imniun抋o modulate action in cerebellum.The IFN- 慳 -Ii positive neurons extensively distributed in nucleus fastigi, nucleus interpostitus, nucleus dentatus of cerebellum. The effect of EA on IFN- 慳?-Ii posi... |
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