Screening Of Virulence Genes Contributing To Streptococcus Suis Type2Translate Across The Blood Brain Barrier

Streptococcus suis is a serious zoonotic diseases, it can cause the pigletsmeningitis, bronchitis, septicemia and arthritis, it also can be infected by the peoplewho direct contact with swine or pork products with the symptom that is septicarthritis, meningitis, endocarditis and toxic shock syndrome. The outcome afterStreptococcus suis infected people are permanent hearing loss or vestibulardysfunction. The two prerequisites that Streptococcus suis invade into the brain arebacteremia and the bacterial could translate across the Blood Brain Barrier. Themechanism of how Streptococcus Suis translate across the Blood Brain Barrier is stillnot yet clear, the aim of this study is to screen the proteins that contribute toStreptococcus suis translate across the Blood Brain Barrier by the interaction of BloodBrain Barrier model and whole-genome phage display library of Streptococcus suis.In this study, porcine brain microvascular endothelial cells and astrocytes wereisolated, as the basic cells of Blood Brain Barrier in vitro model. Porcine brainmicrovascular endothelial cells and astrocytes were co-coltured in the TranswellLiquid Transfer System.4h side leakage experiments and HRP permeability testingverified the Blood Brain Barrier in vitro model quality. The build of Blood BrainBarrier in vitro model filled gaps, especially the interaction between Zoonosespathogenic and pig blood-brain barrier.The genome phage display libraries were build because of the advantages of thephage display technique, the combination of genotypes and protein phenotype inphage display libraries. After three rounds of screen of the interaction betweenStreptococcus suis genome phage display library and Blood Brain Barrier in vitromodel, the clones that could adhere and invade to the cells which composed the BloodBrain Barrier and ultimately change the Blood Brain Barrier permeability wereisolated.14proteins may be involved in the destruction of the blood-brain barrier byStreptococcus suis. There were DNA-directed RNA polymerase, O-acetylhomoserine sulfhydrylase, Collagenase-like protease, Hypothetical protein, Permease, Suilysin,Transposase IS630-Spn1, putative glycogen phosphorylase, Enolase, Sensor histidinekinase, DHH family protein, Phosphopantothenoylcyteine decarboxylase, FBPS,Surface-anchored protein. Suilysin Enolase FBPS and Surface-anchored protein havealresdy been reported to be connected with the pathogenicity of Streptococcus suis. Theprocess that Streptococcus suis translated across the Blood Brain Barrier is affected byseveral factors, the proteins that may contributed to Streptococcus suis translate acrossthe Blood Brain Barrier needs to be further confirmed.